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Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study
Authors:Mazzarelli Gianna  Rindi Laura  Piccoli Paola  Scarparo Claudio  Garzelli Carlo  Tortoli Enrico
Affiliation:Microbiology and Virology Laboratory, Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy.
Abstract:We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.
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