软骨细胞增殖与凋亡在人重组白细胞介素-1β介导的颞下颌关节炎症损伤中的意义

目的:探讨细胞增殖与凋亡在人重组白细胞介素-1B介导的颞颌关节炎症损伤中的作用。方法:选用SD大鼠颞下颌关节腔内反复多次注射rhIL-1B,造成炎症损伤,利用免疫组织化学染色及原位末端标记法,观察炎症损伤过程中髁突软骨细胞增殖及凋亡的变化。结果:注射后1~30 d,实验侧髁突软骨增殖层PCNA阳性指数均较对照侧低,以1~7 d最明显;实验侧髁突软骨细胞凋亡数较对照侧多,主要分布于软骨增殖层及前肥厚层中,以1~7 d 最明显,15 d后凋亡细胞数明显减少,仅见个别凋亡细胞散在分布于软骨浅层。结论:rhIL-1B关节内注射不仅使髁突软骨细胞增殖受抑制,而且细胞凋亡也可能是rhIL-1B致关节损伤的途径之一,细胞增殖与凋亡协同参与了颞下颌关节炎症损伤的病理过程。

Roles of Proliferation and Apoptosis of Chondrocytes in Inflammatory Lesions Mediated by Recombinant Human Interleukin-1β on Condylar Cartilage of Temporomandibular Joint

Objective:The purpose of this studywas to evaluate the role of cell proliferation and apoptosis in the inflammatory lesionme- diated by recombinanthuman interleukin-1Bon condylar cartilage of temporomandibular joint.Methods:The model of inflammatory lesion of temporomandibular jointin SD ratswas established by intraarticularinjectionwith recombinanthuman interleukin-1B. The expressions of proliferating cell nuclear antigen (PCNA) and apoptosis in cartilage were examined by immunohistochemical staining and Tdt-mediated dUTP nick end labelling (TUNEL) during series time of after injection of recombinant human interleukin-1B. Results:The immunostaiing results showed thatPCNAproteinwas expressed in proliferative zone of condylar cartilage, the positive index of PCNA decreased in proliferative zone after injection of recombinant human interleukin-1B1~30 days, and more obviously in 1~7 days. Apoptotic chondrocytes were mainly located in proliferative zone and the prehypertrophic zones, and the apoptotic chondrocytes increased in proliferative and prehypertrophic zones of condylar cartilage (P<0105).Conclusion:These results sug- gest that suppression of proliferating cell nuclear antigen and high apoptosis of chondrocytes may play an important role in the in- flammatory lesion or in the destructive process of temporomandibular joint osteoarthritis.