| 用RAPD技术对我国两个地区马来丝虫DNA多态性的比较分析 |
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| 引用本文： | 边春香,李杰,聂文清. 用RAPD技术对我国两个地区马来丝虫DNA多态性的比较分析[J]. 中国病原生物学杂志, 1999, 0(4) |
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| 作者姓名： | 边春香 李杰 聂文清 |
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| 作者单位： | 大连市第六人民医院!大连116001(边春香)，大连医科大学寄生虫学教研室(李杰，聂文清) |
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| 摘 要： | 本实验采用ＲＡＰＤ 技术对我国湖北谷城和四川乐山两个地区马来丝虫的基因组ＤＮＡ 进行随机扩增，结果得到１５ 个多态片段，其中两个地区丝虫既有相同的条带，又存在差异性条带。这说明两个地区的马来丝虫在基因结构上既有同源性，又存在差异。因而，从分子水平上进一步证实了我国马来丝虫似有种内分化的趋向。
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| 关 键 词： | 马来丝虫 基因组DNA RAPD |
RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF BRUGIA MALAYI FROM TWO ENDEMIC AREAS OF CHINA |
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| Bian Chunxiang Li Jie Nie Wenqing The Sixth People's Hospital of Dalian. RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF BRUGIA MALAYI FROM TWO ENDEMIC AREAS OF CHINA[J]. Journal of Pathogen Biology, 1999, 0(4) |
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| Authors: | Bian Chunxiang Li Jie Nie Wenqing The Sixth People's Hospital of Dalian |
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| Affiliation: | Dalian 116001 |
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| Abstract: | In order to explore whether the parasites of Brugia malayi(B.m.) from Gucheng in Hubei(H) and Leshan in Sichuan(S) have differentiation or not at gene level, we used the technique of random amplified polymorphic DNA(RAPD) to amplify their genomic DNA. As a result, 15 fragments(50-1 000 bp) were obtained i.e. 9 bands(a,b,c,d,e,f,g,h,i) from H area and 6 bands(a,c,d,e,f,g) from S area. Comparison of these bands showed that the parasites of B.m. from two endemic areas had homology, while they also had differences. |
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| Keywords: | Brugia malayi genomic DNA RAPD |
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