Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up. |
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| Authors: | M J Post C Moekoet J Wemer H H van Rooij A J Porsius |
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| Institution: | Department of Pharmacotherapy, Faculty of Pharmacy, University of Utrecht, The Netherlands. |
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| Abstract: | A new method is presented for studying mediator release in isolated peritoneal cells of the rat. Using a superfusion technique, cells can be maintained in a continuously cleared medium. Cells were stimulated with antigen, compound 48/80 or A23187 to characterize this preparation. In addition, inhibitory effects of theophylline, isoprenaline and cromoglycate on compound 48/80-induced histamine release were studied. We conclude that superfusion is a valid alternative to study mediator release from isolated peritoneal cells. |
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