塞来昔布对Lewis肺癌细胞增殖及移植瘤的抑制作用

目的:探讨选择性环氧化酶-2(COX-2)抑制剂塞来昔布对Lewis肺癌细胞株增殖及小鼠皮下移植瘤的影响。方法:常规培养Lewis肺癌细胞,实验分为对照组和塞来昔布用药组。MTT法检测细胞增殖水平,损伤修复实验检测细胞迁移能力;将Lewis肺癌细胞接种于12只C57BL/6小鼠皮下,对照组隔日腹腔注射生理盐水;塞来昔布用药组隔日腹腔注射塞来昔布30 mg/kg,24 d后处死,计算抑瘤率,逆转录聚合酶链反应(RT-PCR)技术检测移植瘤组织中VEGF mRNA表达水平,同时酶联免疫检测小鼠血清中MMP-9浓度。结果:塞来昔布对Lewis肺癌细胞增殖的抑制作用呈时间-剂量依赖性效应(P<0.05)。20μmol/L塞来昔布组细胞迁移速度(0.37±0.03)μm/h明显减慢,与对照组(3.17±0.22)μm/h相比具有显著性差异(P<0.01);在体实验显示,塞来昔布具有明显的抑制Lewis肺癌移植瘤增殖的作用,同时能抑制移植瘤组织中VEGF表达、降低血清MMP-9浓度。结论:塞来昔布可以显著抑制Lewis肺癌细胞的增殖与迁移能力,且对Lewis肺癌移植瘤有明显抑制作用。

Inhibition Effects of Celebrex on the Proliferation of Lewis Lung Cancer Cells and Growth of Transplanted Lewis Lung Carcinoma

Objective: To investigate the effect of selective COX-2 inhibitor Celebrex on the proliferation of Lewis lung cancer cells and growth of transplanted Lewis lung carcinoma.Methods: Lewis lung cancer cells were divided into control and Celebrex group in vitro.The effect of Celebrex at different concentration on the proliferation and migration ability of Lewis lung cancer cells was observed by MTT and wound healing assay.The Lewis lung cancer cell were inoculated subcutaneously in 12 C57BL/6 mice simultaneously,then the mice were randomly divided into control(0.9% NaCl) and Celebrex(30 mg/kg) group. On the 24th day,all the mice were killed,the formation ability of Lewis lung cancer cells was detected.Results: The proliferation of Lewis lung cancer cells decreased with the increased concentration of Celebrex.The migration velocity of Lewis lung cancer cells treated with Celebrex at 20 μmol/L was(3.17±0.22)μm/h in control group versus(0.37±0.03)μm/h in Celebrex groupP<0.01).Celebrex inhibited the growth of transplanted Lewis lung carcinoma in vitro significantly and the inhibited rate was 51.4%(P<0.01). At the same time,VEGF mRNA expression level in transplanted carcinoma tissue and MMP-9 expresion level in serum was significantly down-regulated after the treatment with Celebrex(P<0.05).Conclusion: Celebrex could inhibit Lewis lung cancer cells proliferation,migration and tumor formation ability.