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| 表皮生长因子对类风湿关节炎滑膜细胞的作用 | |
| 引用本文： | 燕太强,吕厚山,药立波. 表皮生长因子对类风湿关节炎滑膜细胞的作用[J]. 中华风湿病学杂志, 2000, 4(5): 270-273 |
| 作者姓名： | 燕太强 吕厚山 药立波 |
| 作者单位： | 北京大学人民医院关节病研究所(燕太强!100044，吕厚山!100044，孙铁铮!100044)，第四军医大学生化及分子生物学教研室(药立波) |
| 基金项目： | 国家自然科学基金!重点项目资助 (39730 430 ) |
| 摘 要： | 目的 观察表皮生长因子（ＥＧＦ）对类风湿关节炎（ＲＡ）滑膜细胞的作用和细胞中有丝分裂素激活的蛋白激酶（ＭＡＰＫ）的激活情况。方法 ３～５代体外培养的ＲＡ滑膜细胞，＾３Ｈ－ＴｄＲ掺入检测ＥＧＦ对细胞ＤＮＡ合成的影响；ＥＧＦ刺激后裂解细胞，收获蛋白，Ｗｅｓｔｅｒｎ ｂｌｏｔ检测ＭＡＰＫ的活化。结果 ＥＧＦ刺激组和对照组每分钟计数值差异有显著性（Ｐ〈０．００１）。ＥＧＦ能引起细胞内明显ＭＡＰＫ活化。结论 |
| 关 键 词： | 表皮生长因子 尿抑胃素 类风湿性关节炎 滑膜 |
Effect of epidermal growth factor on rheumatoid synovial fibroblasts |
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| Abstract: | Objective To determine whether EGF affects DNA synthesis and activates intracellular MAPK in rheumatoid synovial fibroblasts.Methods Cultured rheumatoid synovial fibroblasts were stimulated with exogenous EGF,then investigated 3H-TdR incorporation;and Western blot analysis of cell lysates.Results The incorporation of 3H-TdR was significantly increased upon EGF treatment,meanwhile,immunoblotting revealed that MAPK phosphorylation was greatly increased.Conclusion MAPK is likely to recepter an important component in the cascade of signals that link EGF receptor to EGF eliciting the cellular proliferative response in rheumatoid synovial fibroblasts. |
| Keywords: | Epidermal growth factor-urogastone Arthritis rheumatoid Synovial membrane Fibroblasts Mitogen Protein kinases |
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