当归多糖对豚鼠银屑病样皮损中PCNA表达的影响

目的研究当归多糖对豚鼠耳部银屑病样皮损中细胞增殖性核抗原(PCNA)表达水平的影响。方法50g/L普萘洛尔乳剂外涂豚鼠耳背皮肤,制备银屑病样模型,然后随机分成模型对照组(I)、低剂量组(II)、中剂量组(III)、高剂量组(IV),依次给予生理盐水、100mg/kg,200mg/kg,400mg/kg当归多糖腹腔注射。应用免疫组化法分别检测各组治疗前后皮损中PCNA表达水平。结果造模后豚鼠耳部上皮PCNA阳性表达率与涂药前相比明显增加(P<0.05)。II,III,IV组治疗后皮损中PCNA阳性表达率较治疗前均明显降低(P<0.05)。结论当归多糖对普萘洛尔诱发的豚鼠耳背银屑病样模型有治疗作用,可能与其降低皮损中PCNA表达水平有关。

The Influence of Angelica Polysaccharide on the Expression Levels of PCNA in Psoriasis-like Lesions of Guinea Pigs

Objective To observe the influence of Angelica Polysaccharide on the expression levels of proliferating cell nuclear antigen(PCNA) in psoriasis-like lesions of guinea pigs.Methods 50 g/L propranolol emulsion was applied on ears' back of Guinea pigs to produce the psoriasis-like models,then we randomly divided them into four groups:model control group(I),low-dose group(II),mid-dose group(III) and high-dose group(IV).Normal saline and 100 mg/kg,200 mg/kg,400 mg/kg angelica polysaccharide were injected intra-abdominally by turns.We detected each group the expression levels of PCNA in their skin lesions before and after different treatments by immunohistochemical method.Results The positive rates of PCNA in psoriasis-like lesions increased prominently compared with that of normal guinea pigs(P<0.05).And after injecting angelica polysaccharide in group II,III,IV,the positive rates were decreased greatly than that of before treatment(P<0.05).Conclusion Angelica polysaccharide might have a therapeutic effect on the psoriasis-like lesions in the epidermis of guinea pigs'ears induced by propranolol ointment,which might be related to the decrease the expression of PCNA in lesions.