益气健脾方药对D-半乳糖拟衰小鼠自由基代谢及免疫功能的影响

目的研究益气健脾方药对D-半乳糖(D-gal)所致衰老小鼠自由基代谢及免疫功能的影响.方法采用D-gal复制衰老及脑老化小鼠模型.测定衰老小鼠血清、心、肝组织中总超氧化物歧化酶(T-SOD)活性;心、肝组织中丙二醛(MDA)、脂褐素(LPF)水平;血清白介素-2(IL-2)及胸腺指数,并观察益气健脾方药对上述指标的影响.结果益气健脾方药能明显提高血清、心、肝组织中T-SOD的活性,降低心、肝组织MDA和肝组织LPF含量,并提高血清IL-2含量和胸腺指数.结论抑制脂质过氧化反应,提高抗氧化酶活性,改善免疫功能可能是益气健脾方药延缓衰老的作用机制之一.

The effect of replenishing qi to invigorate the spleen recipe on free radical metabolism and immune function of aging mice induced by D-gal

Objective To explore The effect of replenishing qi to invigorate the spleen recipe on free radical metabolism and immune function of aging mice induced by D-gal. Methods Total superoxide dismutase(T-SOD) activity in serum, heart and liver tissue, ma londialdehyde(MDA) and lipofuscin( LPF) levels in heart and liver tissue, and serum IL-2 and thymus indexes were determined in aging mice model induced by D-gal. The effect of replenishing qi to invigorate the spleen recipe on the above indexes was observed. Results The replenishing qi to invigorate the spleen recipe could significantly increase the T-SOD activity in serum, heart and liver tissue; decrease MDA content in heart and liver and LPF content in liver; enhance serum IL-2 content and thymus indexes. Conclusions To inhibit lipid peroxida-tion, increase activity of antioxidase and improve immune function might be one of mechanisms of replenishing qi to invigorate the spleen recipe against aging.