Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components |
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Authors: | E Fowler N Cheng |
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Affiliation: | Cancer Research Center, Department of Bacteriology and Immunology, and Genetics Curriculum, University of North Carolina at Chapel Hill, NC 27514, U.S.A. |
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Abstract: | A solid phase radioimmunoassay has been compared with an enzyme-linked immunosorbent assay (ELISA) for efficacy in measuring anti-chromatin antibodies. The low backgrounds achieved with the radioimmunoassay method produced a high signal-to-noise ratio and enabled detection of the human test antiserum at a dilution of 1:102,400. By contrast, the ELISA could detect the same antiserum only at a dilution of 1:3200 and above. The radioimmunoassay was consistently more sensitive than the ELISA for detection of anti-chromatin antibodies in a number of human and mouse sera and ascites fluid containing a monoclonal antibody. Factors affecting sensitivity in both assays are discussed. |
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Keywords: | radioimmunoassay ELISA antibodies chromatin components ELISA enzyme linked immunosorbent assay RIA radioimmunoassay Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid BSA bovine serum albumin |
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