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| 高脂血症对大鼠主动脉内皮细胞Bax、Bcl-2蛋白表达的影响 | |
| 引用本文： | 蔡辉,赵凌杰,赵智明,李蓉,袁爱红. 高脂血症对大鼠主动脉内皮细胞Bax、Bcl-2蛋白表达的影响[J]. 微循环学杂志, 2011, 21(4): 1-3,7,10,4 |
| 作者姓名： | 蔡辉 赵凌杰 赵智明 李蓉 袁爱红 |
| 作者单位： | 南京军区南京总医院中西医结合科,南京,210002 |
| 摘 要： | 目的:探讨高脂血症SD大鼠主动脉内皮细胞凋亡蛋白Bax、Bcl-2表达的变化及其诱导内皮细胞凋亡的可能机制。方法:清洁级大鼠17只,随机分为高脂血症模型组(8只)和对照组(9只),分别以高脂饲料和普通饲料喂养16周后,检测血脂水平,采用免疫组织化学方法检测两组大鼠主动脉内皮细胞Bax、Bcl-2的表达,同时用HE染色和电镜观察主动脉组织形态学改变。结果:与对照组相比,模型组大鼠血清甘油三酯(TG)、总胆固醇(TC)水平升高(P<0.01),主动脉内皮细胞Bax蛋白表达明显增加(P<0.01),Bcl-2蛋白表达明显减少(P<0.01),Bcl-2/Bax比值明显降低(P<0.01);模型组主动脉内膜增厚和平滑肌细胞增殖,内皮组织部分脱落,内皮细胞胞膜破裂,胞质脱失,核膜不完整。结论:高脂血症可引起大鼠血管内皮细胞凋亡蛋白Bax、Bcl-2水平及比值改变,加重血管内皮损伤,促进细胞凋亡,加速动脉粥样硬化进程。 |
| 关 键 词： | 高脂血症 内皮细胞 凋亡蛋白 Bax/Bcl-2 动脉粥样硬化 |
The Influence of Hyperlipoidemia on Bax and Bcl-2 Protein Expression in Rat Aortic Endothelial Cells |
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| Cai Hui,Zhao Lingjie,Zhao Zhiming,et al. The Influence of Hyperlipoidemia on Bax and Bcl-2 Protein Expression in Rat Aortic Endothelial Cells[J]. Chinese Journal of Microcirculation, 2011, 21(4): 1-3,7,10,4 | |
| Authors: | Cai Hui Zhao Lingjie Zhao Zhiming et al |
| Affiliation: | Cai Hui,Zhao Lingjie,Zhao Zhiming,et al Department of Integrated Traditional Chinese and Western Medicine,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002 |
| Abstract: | Objective:To study the change of Bax and Bcl-2 protein expression in hyperlipoidemia rat aortic endothelial cells and the possible mechanism of promoting endothelial cells apoptosis.Method:17 male Sprague Dawley rats were randomly divided into hyperlipoidemia model group(n=8) and control group(n=9),respectively raised for 16w with high-fat diets and normal diets.Serum lipid level of all rats was measured and apoptosis protein of Bax and Bcl-2 on the aortic endothelium cell were analyzed by immunohistochemic... |
| Keywords: | Hyperlipoidemia Endothelial cells Apoptosis protein Bax/Bcl-2 Atherosclerosis |
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