| 安宫牛黄丸中小檗碱的HPLC法测定 |
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| 引用本文： | 林似兰,赵陆华,王义明,董善士,安登魁.安宫牛黄丸中小檗碱的HPLC法测定[J].药学学报,1989,24(1):48-52. |
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| 作者姓名： | 林似兰 赵陆华 王义明 董善士 安登魁 |
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| 作者单位： | 中国药科大学，中国药科大学，中国药科大学，中国药科大学，中国药科大学 南京 210009，南京 210009，南京 210009，南京 210009，南京 210009 |
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| 摘 要： | 本文报道用HPLC法测定黄连及含黄连中成药安宫牛黄丸中小檗碱型生物碱。实验结果表明选用硅胶柱为固定相,以醋酸乙酯—甲酸—乙醇(15:3:2)为流动相,能使样品中四种小檗碱型的生物碱获得最佳分离。用此法测得不同厂家生产的安宫牛黄丸中盐酸小檗碱的含量为0.331～0.456%,平均回收率为97.23%,变异系数为1.2%。
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| 关 键 词： | 盐酸小檗碱 黄连 安宫牛黄丸 高效液相色谱法 |
| 收稿时间： | 1987-11-16 |
DETERMINATION OF BERBERINE IN ANGONG NIUHUANG WAN BY HPLC |
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| SL Lin,LH Zhao,YM Wang,SS Dong and DK An.DETERMINATION OF BERBERINE IN ANGONG NIUHUANG WAN BY HPLC[J].Acta Pharmaceutica Sinica,1989,24(1):48-52. |
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| Authors: | SL Lin LH Zhao YM Wang SS Dong and DK An |
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| Abstract: | A normal phase liquid chromatographic method was developed for the determination of berberine-type alkaloids in rhizoma Coptidis and traditional Chinese medicine--Angong Niuhuang Wan. Experimental evidences indicate that a normal phase system of a silica column (3.9 x 250 mm) as stationary phase with an eluent of ethyl acetate-formic acid-ethanol (15:3:2) as mobile phase can separate excellently (R5 greater than 1.5) four berberine-type alkaloids in the samples tested. According to the results obtained with this method the percent content of berberine hydrochloride in some Angong Niuhuang Wan from different factories and different batches was 0.331-0.456% and the average recovery was 97.23%; coefficient of variation was 1.2%. |
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| Keywords: | Berberine hydrochloride Rhizoma Coptidis Angong Niuhuang Wan HPLC |
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