红鲫Sox1和Sox11b基因保守区的克隆与表达分析

目的 进一步理解鱼类Sox基因的进化和表达方式.方法 采用兼并引物PCR,从红鲫基因组DNA中克隆、测序和分析Sox基因.采用RT-PCR,研究Sox基因在红鲫13种不同组织中的表达.结果 克隆和测序了红鲫Sox1-1、Sox1-2、Sox1-3、Sox11b1-1、Sox11b1-2和Sox11b2基因的HMG盒区序列.Sox1-1、Sox1-2和Sox1-3编码相同的氨基酸,在肝、脾、肾、心脏和脑组织中均有不同程度表达, 在脑组织中检测到大量的转录物.Sox11b1-1和Sox11b1-2编码相同的氨基酸,系统发生树显示,红鲫Sox11b1和Sox11b2基因与斑马鱼Sox11b基因聚在一起,而远离斑马鱼Sox11a基因.在下丘脑和小脑中检测到Sox11b1-1和Sox11b1-2基因转录物,而没有检测到Sox11b2基因转录物.结论 Sox1和Sox11b基因在进化过程中发生了复制.Sox1基因可能在神经系统中起着重要的作用.Sox11b1-1、Sox11b1-2和Sox11b2有相似而不同的表达方式.

Isolation and Expression Analysis of HMG-box Motifs of Sox1 and Sox11b Genes in the Red Crucian Carp

Objective To further understand the evolution and expression pattern of Sox genes in fish.Methods Genomic DNA from the red crucian carp was used as the template for PCR amplification using degenerate primers PCR.PCR products were cloned,sequenced, and analyzed.The expression patterns of Sox genes in 13 adult tissues of the red crucian carp were analyzed by RT-PCR.Results Six HMG-box sequences of Sox1-1,Sox1-2,Sox1-3,Sox11b1-1,Sox11b1-2 and Sox11b2 were cloned and sequenced.Sox1-1,Sox1-2 and Sox1-3 shared th...