红花黄色素对体外循环肺再灌注损伤的保护作用

目的:探索红花黄色素(SY)对体外循环(cardiopulmonary bypass,CPB)后肺再灌注损伤的保护作用。方法:将24只小型猪随机分为四组,每组6只。假手术组(A组);CPB对照组(B组);红花黄色素0.26 g/kg组(C组);红花黄色素0.37 g/kg组(D组)。CPB前、肺再灌注30、60、120和240min采集相应的动脉血(或肺静脉血),检测丙二醛(MDA)含量、肿瘤坏死因子(TNF-α)、中性粒细胞和单核细胞,再灌注240min后,取右肺下叶相同部位肺组织,测定湿干质量比值。结果:与A组比较,B组的MDA和TNF-α再灌注后显著增高(P0.05),中性粒细胞和单核细胞也明显升高(P0.05);与B组比较,C,D组的MDA和TNF-α有所降低,尤其在肺再灌注240min(P0.01),单核细胞显著降低(P0.05);D组与B组相比较,中性粒细胞显著降低(P0.05),湿干重质量比值明显减少(P0.05);与C组比D组的TNF-α降低明显(P0.05)。结论:红花黄色素对CPB肺损伤具有缓解作用,高剂量组较低剂量组效果更加明显。

The effect of safflor yellow on lung injury with cardiopulmonary bypass in pigs

Objective：To investigate the effect of safflor yellow on lung injury after cardiopulmonary bypass （CPB） in pigs.Methods：All miniature pigs were randomly divided into 4 groups：sham group （A group）,CPB model group（B group）,safflor yellow 0.26 g / kg group（C group）,safflor yellow 0.37 g / kg group（D group）.Blood samples were collected before CPB and reperfusion 30,60,120 and 240 min.Malondialdehyde （MDA）,tumor necrosis factor （TNF-α）,neutrophils and monocytes were detected.Dry/wet weight ratio of the right lower lobe lung tissue were defined after reperfusion 240min.Results：Compared with A group,MDA and TNF-o in B group were significantly higher after reperfusion （P ＜ 0.05）,neutrophils and monocytes in B group increased significantly as reperfusion （P ＜ 0.05）.Compared with B group,MDA and TNF-α in C,D group were significantly lower （P ＜ 0.05）.With B group,Dry/wet weight ratio in D group was lower （P ＜ 0.05）.Conclusion：Safflor yellow can protect lung injury during cardiopulmonary bypass.