| 人类醛糖还原酶类似蛋白1 siRNA慢病毒载体的构建及功能鉴定 |
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| 引用本文： | 祖旭宇,蔡秋香,曹德良,廖端芳. 人类醛糖还原酶类似蛋白1 siRNA慢病毒载体的构建及功能鉴定[J]. 南华大学学报(医学版), 2009, 37(5): 495-498 |
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| 作者姓名： | 祖旭宇 蔡秋香 曹德良 廖端芳 |
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| 作者单位： | 祖旭宇(南华大学,第一附属医院,临床研究所,湖南,衡阳,421001);蔡秋香,廖端芳(南华大学,药物药理研究所);曹德良(美国南伊利偌州大学,医学院,肿瘤研究所) |
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| 基金项目： | 中国博士后科学基金资助 |
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| 摘 要： | 目的构建人类醛糖还原酶类似蛋白1（ARL-1）siRNA幔病毒载体。方法设计针对于ARL-1Mma的siRNA，将siRNA连接于pENTRTM／U6载体，经测序确认后，通过免疫印迹和AI也-1酶活性鉴定验证ARL-1siRNA慢病毒载体的功能。结果ARL-1siRNA慢病毒载体能有效地沉默HCT8细胞内ARL-1的表达，降低HCT8细胞内ARL-1的酶活性。结论获得了能有效抑制细胞内ARL-1蛋白表达的ARL-1siR-NA慢病毒。
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| 关 键 词： | ARL-1 siRNA 慢病毒载体 HCT8细胞 |
Construction of ARL-1 siRNA Lentivirus and Identification of its Function |
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| ZU Xu-yu,CAI Qiu-xiang,CAO De-liang,et al. Construction of ARL-1 siRNA Lentivirus and Identification of its Function[J]. Journal of Nanhua University(Medical Edition), 2009, 37(5): 495-498 |
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| Authors: | ZU Xu-yu CAI Qiu-xiang CAO De-liang et al |
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| Affiliation: | ZU Xu-yu,CAI Qiu-xiang,CAO De-liang,et al(Clinical Institution,the First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,China) |
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| Abstract: | Objective To obtain ARL-1 siRNA Lentivirus.Methods siRNA targeting ARL-1 Mrna was designed and ligated with pENTRTM/U6 plasmid.After verification by sequence,HCT8 cells was infected with ARL-1 siRNA Lentivirus,and the Western blot and enzyme activity assay were used for ARL-1 siRNA Lentivirus function confirmation. Results ARL-1 siRNA Lentivirus could greatly knockdown the expression of ARL-1 and decrease the ARL-1 enzyme activity in HCT8 cells. Conclusion Lentivirus with expression of siRNA targeting to AL... |
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| Keywords: | ARL-1 siRNA |
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