更年健上调老年雌性大鼠下丘脑雌激素受体mRNA表达对P物质和β-内啡肽的影响 |
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引用本文: | 步世忠,孙梅,张沅,张永莲,俞瑾.更年健上调老年雌性大鼠下丘脑雌激素受体mRNA表达对P物质和β-内啡肽的影响[J].中国中西医结合杂志,1998,18(1):28-31. |
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作者姓名: | 步世忠 孙梅 张沅 张永莲 俞瑾 |
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基金项目: | 国家中医药管理局基金!(No9221A5) |
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摘 要: | 目的:探讨中药复方更年健上调老年雌性大鼠下丘脑雌激素受体( ER)和 ER信使核糖核酸(mRNA)对P物质(SP)和β-内啡肽(β-EP)的影响。方法:建立自然衰老的雌性大鼠模型,用放射配体结合分析法检测 ER,用 Northern blot分析 ERmRNA的改变,用放射免疫分析法检测 SP和β-EP含量。结果:老年雌性大鼠性减退期下丘脑 ER和 ERmRNA水平随血清E2水平的下降较性成熟期显著下降,而更年健则使下降了的ER和ERmRNA水平明显提高,并通过上调下丘脑ERmRNA使SP明显下降,而β-EP则显著提高,与提高体内雌激素水平具有同样的效果。结论:更年健可增加RR在下丘脑的表达以增强雌激素的生物学效应,同时降低下丘脑 SP水平而提高 β-EP的含量。
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关 键 词: | 更年健 雌激素 mRNA P物质 内啡肽 更年期综合征 |
Effect of Gengnianjian on up regulated estrogen receptor mRNA to substance P and beta-endorphin in hypothalamus of aging female rats] |
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Authors: | S Bu M Sun Y Zhang |
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Institution: | Obstetric and Gynecdogy Hospital, Shanghai Medical University, Shanghai(200011). |
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Abstract: | OBJECTIVE: To investigate the effect of Chinese herbal formula Gengnianjian on estrogen receptor (ER), ERmRNA substance P (SP) and beta-endorphin (beta-EP) in the hypothalamus of aging female rats. METHODS: ER proteins were determined by radioligand binding assay, ER mRNA was analyzed by Northern blot analysis and the levels of beta-EP and SP were detected by immunohistochemistry. RESULTS: In aging rats, lowered ER and ER mRNA in the hypothalamus were still up-regulated by low serum E2 level, but not in rats of after the age of 21 months; Gengnianjian significantly increased ER protein level and ER mRNA expression, decreased SP and increased the level of beta-EP in the hypothalamus in aging female rats before 21 months of age, without changing of serum E2 level. In EB group, serum E2 and beta-EP levels increased and SP level decreased but ER expression suppressed in the hypothalamus except after 21 months. CONCLUSION: Enhance estrogen bioactivity can decrease the level of SP and increase the level of beta-EP in hypothalamus of aging female rats. Herbal formula Gengnianjian increase-EP level and decrease SP level by raising the level of ER and ER mRNA. |
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