| 罗格列酮对高糖培养肾小球系膜细胞胞外基质产生及降解的影响 |
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| 引用本文： | 倪连松,金洁娜,郑景晨,沈飞霞.罗格列酮对高糖培养肾小球系膜细胞胞外基质产生及降解的影响[J].中国临床药理学与治疗学,2008,13(10). |
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| 作者姓名： | 倪连松 金洁娜 郑景晨 沈飞霞 |
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| 作者单位： | 温州医学院附属第一医院内分泌科,温州,325000,浙江 |
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| 基金项目： | 温州市科技局资助项目，省留学回国基金 |
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| 摘 要： | 目的:探讨罗格列酮(rosiglitazone maleate)对糖尿病肾病的保护机制。方法:大鼠肾小球系膜细胞分别培养在正常糖浓度(5.5mmol/L),高糖浓度(25mmol/L)及25mmol/L葡萄糖+20μmol/L罗格列酮的培养液中。CCK-8测定系膜细胞增殖;ELISA法检测培养上清液Ⅳ型胶原(Col-Ⅳ)、纤维连接蛋白(FN)、转化生长因子-β1(TGF-β1)、基质金属蛋白酶组织抑制因子-1(TIMP-1);明胶酶谱法检测培养上清液基质金属蛋白酶-2,9(MMP-2,9)的活性。结果:高糖组系膜细胞较正常对照组出现增殖增加,合成基质蛋白Col-Ⅳ、FN增多;MMP-2及MMP-9活性下降;TIMP-1含量增加;TGF-β1分泌增加。与高糖组比较,罗格列酮干预后能逆转上述变化。结论:罗格列酮能抑制高糖培养的系膜细胞增殖,减少胞外基质合成,增加胞外基质的降解。
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| 关 键 词： | 糖尿病肾病 罗格列酮 肾小球系膜细胞 |
Effects of rosiglitazone on the production and degradation of extracel-lular matrix of glomerular mesangial cells incubated with high concentration of glucose |
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| NI Lian-song,JIN Jie-na,ZHENG Jing-chen,SHEN Fei-xia.Effects of rosiglitazone on the production and degradation of extracel-lular matrix of glomerular mesangial cells incubated with high concentration of glucose[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2008,13(10). |
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| Authors: | NI Lian-song JIN Jie-na ZHENG Jing-chen SHEN Fei-xia |
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| Institution: | NI Lian-song; JIN Jie-na; ZHENG Jing-chen; SHEN Fei-xia Department of Endocrinology; the First Affiliated Hospital of Wenzhou Medical College; Wenzhou 325000; Zhejiang; China; |
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| Abstract: | AIM:To investigate the protection mechanisms of rosiglitazone on diabetic nephropathy.METHODS:Rat mesangial cells(MC) were incubated in media containing 5.5 mmol/L normal control glucose,25 mmol/L high concentration glucose,25 mmol/L glucose +20 μmol/L rosiglitazone maleate.Cells proliferation were assessed by CCK-8.Synthesis of fibronectin(FN),type Ⅳ collagen(Col-Ⅳ),transforming growth factor-β1(TGF-β1) and matrix metalloproteinase inhibitor-1(TIMP-1) in supernatant were determined by ELISA method,the acti... |
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| Keywords: | diabetic nephropathy rosiglitazone mesangial cells |
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