紫外衍生-高效液相色谱法测定保健食品和饮料中的三氯蔗糖

目的 建立保健食品和饮料中三氯蔗糖的柱前紫外衍生-高效液相色谱测定方法。方法 样品经水浸提、离心后，取样液与苯甲酰氯在碱性介质中反应生成具有紫外吸收的衍生产物，然后用高效液相色谱法分析。色谱条件:分离柱为C18柱（250 mm×4.6 mm，4 μm)，流动相为甲醇-水( 95∶5，V/V)，检测波长为232 nm。以保留时间定性，标准曲线法定量。 结果 三氯蔗糖在0.05～1.00 μg范围内，与色谱峰面积有良好的线性关系（r=0.999 8），方法检出限为0.001 25 μg。方法加标回收率为97.4%～102.0%，相对标准差均小于5.0%，方法的日内精密度和日间精密度分别为1.52%和4.04%。结论 该方法简便快速、分离效果好，准确度高，不需要特殊检测器，适用于保健食品和饮料中三氯蔗糖的快速测定。

Determination of Sucralose in Foods and Beverages by Ultraviolet Derivatization-High Performance Liquid Chromatography

Objective To establish a method for determination of sucralose in foods and beverages using high performance liquid chromatography. Methods Sucralose was extracted with water and centrifuged, and then derivatized with benzoyl chloride in alkaline medium. The ultraviolet absorbing derivatives were separated on a Hydro-RP 80 C18 column （250 mm×4. 6 mm, 4 μm, Synergi) using methanol-water (95∶5,V/V) as mobile phase with UV detection at 232 nm．Results A good correlation (correlation coefficient=0.999 8) between detected and actual sucralose was achieved in the range of 0.05 to 1.00 μg. The detection limit of sucralose was 0.001 25 μg. The recoveries were in the range from 97.4% to 102.0% with relative standard deviations of less than 5.0%. The intraday and interday relative standard deviations of the method were 1.52% and 4.04%, respectively. Conclusion This method is simple, rapid, and accurate without the need of special detectors, and it can be used for rapid determination of sucralose in foods and beverages.