| EB病毒特异性DNA酶的纯化制备 |
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| 引用本文： | 李泽卿,田勇泉,肖健云,赵素萍,陶正德.EB病毒特异性DNA酶的纯化制备[J].中国耳鼻咽喉颅底外科杂志,2000,6(1):28-31. |
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| 作者姓名： | 李泽卿 田勇泉 肖健云 赵素萍 陶正德 |
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| 作者单位： | 1. 南京军区南京总医院耳鼻咽喉科，江苏南京 210002
2. 湖南医科大学附属湘雅医院耳鼻咽喉科，湖南长沙 410008 |
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| 摘 要： | 目的 纯化ＥＢ病毒特异性ＤＮＡ酶（ＥＢＶ－ＤＮａｓｅ）为鼻咽癌血清学诊断方法－血清ＥＢＶ－ＤＮａｓｅ抗体的检测奠定基础。方法 采用多重纤维素柱层析对用联合化学诱导的方法制备的ＥＢＶ－ＤＮａｓｅ粗提物进行纯化。 结果 在ＳＤＳ－ＰＡＧＥ检测中，从粗提物到纯化终产物，蛋白质带逐渐减少直至单一条带。其分子量为５２ｋＤ，其对应的每豪克蛋白质酶单位分别为５．４６，４９．１９，１３４．８６，３９１，２５，９６
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| 关 键 词： | DEAE纤维 EBV－DNase DNA酶 鼻咽肿瘤 |
| 文章编号： | 1007-1520(2000)01-0028-04 |
| 修稿时间： | 1999年8月14日 |
Pupification of Epstein-Barr virus-specific DNase |
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| LI Ze qing,TIAN Yong quan,XIAO Jian yun,et al..Pupification of Epstein-Barr virus-specific DNase[J].Chinese Journal of Otorhinolaryngology-skull Base Surgery,2000,6(1):28-31. |
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| Authors: | LI Ze qing TIAN Yong quan XIAO Jian yun |
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| Abstract: | Objective To purify Epstein-Barr virus-specific DNase (EBV-DNase) for further establishment of detection of EBV-DNase antibody in nasopharyngeal carcinoma (NPC) sera. Methods The EBV-DNase extract obtained through combined chemical induction was purified with multistage of gradient linear cellulose column chromatography. Results SDS-PAGE showed that the protein bands were gradually decreased to a single one with a molecular weight of 52kD from the crude extract to the terminal purified product. The immunoradiological determination showed that the units per milligram protein (U/mg) were 5.46, 49.19, 134.86, 391.25 and 967.78 respectively. The enzyme activity was enhanced by 177.2 folds. Conclusion Gradient linear cellulose column chromatography can satisfactorily purify EBV-DNase from the crude cellular extract. |
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| Keywords: | EBV-DNase/isol NASOPHARYNGEAL NEOPLASMS/diag CHROMATOGRAPHY DEAE-CELLULOSE |
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