Sulfur mustard causes oxidants/antioxidants imbalance through the overexpression of free radical producing-related genes in human mustard lungs |
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| Affiliation: | 1. Department of Chemistry and Physics, Area of Analytical Chemistry, University of Almería, La Cañada de San Urbano, Almería 04120, Spain;2. Campus de Excelencia Internacional Agroalimentario CeiA3, La Cañada de San Urbano, Almería 04120, Spain;3. Département de Chimie, Laboratoire de synthèse organique appliquée, Université Oran1, Oran, Algeria;2. Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Granada, Granada, Spain;3. Competence Centre on Health Technologies, University of Tartu, Tartu, Estonia |
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| Abstract: | The aim of this study is to analyze oxidative stress (OS) and changes in expression of reactive oxygen species (ROS) producing-related genes in mustard lungs. Human lung biopsies provided from controls (n = 5) and sulfur mustard (SM)-exposed patients (n = 6). Changes in expression of dual oxidases (DUOXs), aldehyde oxidase 1 (AOX1), thyroid peroxidase (TPO), myeloperoxidase (MPO) and eosinophil Peroxidase (EPO) were measured using RT2 Profiler™ PCR Array. OS was evaluated by determining bronchoalveolar lavage fluids (BALF) levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Higher TAC value was observed in BALF of controls compared with patients (0.138 ± 0.02683 μmol/l vs 0.0942 ± 0.01793 μmol/l), whereas a significant increase in MDA concentration was found in patients (0.486 ± 0.04615 nmol/l vs 0.6467 ± 0.05922 nmol/l). All ROS producing-related genes were overexpressed in the order AOX1> MPO> DUOX2> DUOX1> TPO> EPO. Upregulation of these genes may be a reason for overproduction of ROS, oxidants/antioxidants imbalance, OS and respiratory failures in mustard lungs. |
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| Keywords: | Lung tissue Oxidative stress Oxidants/antioxidants imbalance ROS producing-related genes Sulfur mustard |
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