Mumps outbreaks in a highly vaccinated population: Investigation of a neutralization titre against the current circulating wildtype genotype G5 mumps virus |
| |
| Affiliation: | 1. Department of Biomedical Science and Humana Oncology, Aldo Moro University of Bari, Italy;2. Department of Transfusion Medicine/Blood Bank, Policlinico General Hospital, Bari, Italy;3. Hygiene Department, Policlinico General Hospital, Bari, Italy;1. Department of Pediatrics, Iizuka City Hospital, Iizuka, Japan;2. Nagai Pediatric Clinic, Takamatsu, Japan;3. Ito Pediatric Clinic, Kurume, Japan;4. National Hospital Organization, Mie Hospital, Tsu, Japan;5. Laboratory of Viral Infection, Kitasato Institute for Life Sciences, Tokyo, Japan;6. The Committee of Immunization of the Society of Ambulatory and General Pediatrics of Japan, Japan;1. Multidisciplinary Unit for Biomedical Research (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal;2. Public Health Research Centre, National School of Public Health, Universidade NOVA de Lisboa, Avenida Padre Cruz, 1600-560 Lisboa, Portugal;3. Epidemiology Research Unit, Instituto de Saúde Pública (ISPUP), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal;4. Laboratory of Microbiology, Department of Biological Sciences, Faculty of Pharmacy of University of Porto, Rua de Jorge Viterbo Ferreira n.°228, 4050-313 Porto, Portugal;5. Health Research Unit of School of Health Sciences, Polytechnic Institute of Leiria, Campus 2 - Morro do Lena - Alto do Vieiro, Apartado 4137, 2411-901 Leiria, Portugal;1. Department of Surgery, University of Arizona College of Medicine, Tucson, Arizona;2. Department of Surgery, Indiana University School of Medicine, Fort Wayne, Indiana;3. University of Houston, School of Law, Houston, Texas;4. Department of Surgery, Lutheran Hospital of Indiana, Fort Wayne, Indiana |
| |
| Abstract: | BackgroundMumps outbreaks continue to occur globally, despite high levels of uptake of the mumps vaccine.ObjectivesIn order to address immunity to the current circulating wildtype virus, we sought to determine a mumps G5 specific IgG quantitative value which correlates with genotype G5 specific neutralization ability in vitro.Study designSera from 199 individuals including controls and acute mumps cases were assessed for mumps specific IgG titres using five different enzyme immunoassays coated with antigen from different mumps virus strains. A subset of 66 sera was also assessed for in vitro neutralizing antibody against a contemporary circulating genotype G5 mumps virus.ResultsFor all the different antigenic targets, mumps specific IgG titres were higher in patients following acute mumps infection compared to controls. In acute mumps infected patients, females showed significantly higher serum titres of anti-G5 IgG compared to males (p < 0.05). Furthermore, control males did not show any change in G5 specific IgG with increasing age whereas females show a progressive rise in titre. Linear regression analysis revealed a significant association between the mumps G5 specific IgG levels in the EIA and the in vitro neutralization titres (r2 = 0.59).ConclusionsSpecific IgG to the current circulating genotype G5 mumps strain showed significantly lower titres in males which supports our previous observation that there is a male gender bias in cases of acute mumps infection. Furthermore, in this preliminary study, the data indicate that genotype G5 specific IgG levels of >40 RU/ml are required for neutralization capability to be observed in vitro. |
| |
| Keywords: | Mumps outbreaks Mumps virus-specific IgG G5 mumps virus Neutralization Gender bias |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
