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Dietary vitamin C reduced mercury contents in the tissues of juvenile olive flounder (Paralichthys olivaceus) exposed with and without mercury
Affiliation:1. Department of Coal Chemistry and Environmental Sciences, Poland;2. Department of Fuels Technology, Faculty of Energy and Fuels, AGH – University of Science and Technology in Kraków, Al. A. Mickiewicza 30, 30-059 Kraków, Poland;3. Výzkumné Energetické Centrum, Technická Univerzita Ostrava, 17 Listopadu 15/2172, 708-33 Ostrava–Poruba, Czech Republic;1. Indian Council of Agricultural Research, Central Institute of Fisheries Education, Panch Marg, Yari Road, Versova, Andheri West, Mumbai 400061, India;2. Indian Council of Medical Research Institute, National for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai 400012, India
Abstract:
A 2 × 3 factorial design was employed to evaluate the effects of dietary vitamin C (l-ascorblyl-2-monophosphate, C2MP) levels on growth and tissue mercury (Hg) accumulations in juvenile olive flounder, Paralichthys olivaceus. Six experimental diets with two levels of mercuric chloride (0 or 20 mg HgCl2/kg diet) and three levels of vitamin C (0, 100, or 200 mg C2MP/kg diet) were added to the basal diet. At the end of 6 weeks feeding trial, in presence or absence of dietary Hg, fish body weight gain, specific growth rate, feed efficiency, protein efficiency ratio and whole body lipid content were increased in a dose-dependent manner as dietary vitamin C level increased in the diets. Interestingly, fish fed 100 or 200 mg C2MP/kg diets showed significant interactive effects on reducing Hg content in kidney tissue. These results revealed that dietary vitamin C as 100 or 200 mg C2MP/kg diet had protective effect against Hg accumulation in juvenile olive flounder.
Keywords:Vitamin C  Mercuric chloride  Growth  Tissue mercury content  Olive flounder
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