Molecular detection and genetic characterization of circulating measles virus in northern Italy |
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| Affiliation: | 1. Operative Unit of Clinical Microbiology, Laboratory of Virology, St. Orsola-Malpighi University Hospital, Via Massarenti 9, 40138 Bologna, Italy;2. Public Health Unit Emilia-Romagna Region, Viale Aldo Moro 21, 40127 Bologna, Italy;3. Unit of Medical Genetics, Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy;4. Unit of Medical Genetics, St. Orsola-Malpighi University Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy;5. Department of Specialised, Experimental, and Diagnostic Medicine, Operative Unit of Clinical Microbiology, Laboratory of Virology, St. Orsola-Malpighi University Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy;1. Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan;2. Department of Microbiology, Nanchang Center for Disease Control and Prevention Nanchang, Jiangxi, China;3. Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan;4. Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan;5. College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia;6. Institute of Veterinary Medicine, Zaisan, Ulaanbaatar, 17024, Mongolia;7. Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565-0781, Japan;1. Kenya Agricultural and Livestock Research Organization (KALRO), Biotechnology Research Institute (BioRI), P. O. Box 362, Kikuyu, Kenya;2. Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000–00200, Kenya;3. Kenya Food Crop Research Institute, P. O. Box 30148, Nairobi, Kenya;1. WHO European Regional Reference Laboratory for Measles and Rubella, Department of Infection and Immunity, Luxembourg Institute of Health, 29 rue Henri Koch, L-4354 Esch-sur-Alzette and Laboratoire National de Santé, 1 rue Louis Rech, L-3555 Dudelange, Luxembourg;2. Direction de la Santé, Division de l''Inspection Sanitaire, 20 rue de Bitbourg, L-1273 Luxembourg-Hamm, Luxembourg;1. Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan;2. Shiranaga Animal Hospital, 2-12-18 Sakuragi, Shunan, Yamaguchi 745-0806, Japan;3. Morinoinuneko Animal Hospital, 22-3 Tsunakizaka, Imosawa, Aoba-ku, Sendai, Miyagi 989-3212, Japan;4. Institute of Natural and Environmental Science, University of Hyogo, 940 Sawano, Aogaki-cho, Tamba, Hyogo 669-3842, Japan;5. Hikiiwa Park Center, 1629 Inari-cho, Tanabe, Wakayama 646-0051, Japan;6. Department of Dermatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami- Kogushi, Ube, Yamaguchi 755-8505, Japan |
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| Abstract: | BackgroundLaboratory diagnosis of measles virus (MV) infection and genetic characterization of circulating MV play an essential role in measles surveillance, allowing proper interventions to interrupt endemic transmission.ObjectivesWe describe results obtained using serological and molecular methods to confirm MV infection among suspected cases reported in a large region in the north of Italy during 2010–2014 and the genotyping of the MV strains detected.Study designThree hundred seventy-two samples (361 urine and 11 oral fluids) were tested for MV-RNA detection. In 281 cases, the serological results for MV-IgM detection were also available.ResultsA total of 276 cases were classified as confirmed measles and MV-RNA detection resulted positive for 239/276 cases.Nucleotide sequence analysis revealed sporadic cases of genotypes D9 and different circulations of endemic MV strains (D8, D4 and B3).ConclusionsThis data suggests that there is still an unvaccinated part of the population maintaining the endemic circulation of MV in Italy. |
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| Keywords: | Measles virus Molecular methods Genetic characterization Serological diagnosis Sampling time |
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