p 16基因表达及高危 HPV 感染在宫颈癌中相关性的研究

目的：探讨 p16基因的表达和高危 HPV 感染（HR-HPV）在宫颈癌发生、发展中的意义。方法运用实时荧光定量PCR（FQ-PCR）检测 p16基因在宫颈癌组织、上皮肉瘤组织（CIN）、正常宫颈组织中的表达，原位杂交检测上述标本中 HR-HPV感染。结果p16基因在宫颈癌组织、CIN、正常宫颈组织中的表达水平分别为0．79±0．34、0．70±0．36、0．26±0．21，差异具有统计学意义（P ＜0．05）。p16基因的表达水平在不同临床分期之间的差异具有统计学意义（P ＜0．05），但肿瘤分化程度、淋巴结转移、肿瘤大小等其他临床病理特征对其无显著影响（P ＞0．05）；HR-HPV 阳性患者的 p16基因的表达水平明显高于 HR-HPV阴性患者，差异有统计学意义（P ＜0．05）。结论p16基因在宫颈癌中呈高表达状态，并且与 HR-HPV 感染密切相关，基因的高表达可能在宫颈癌的发生和发展中发挥重要作用。

Correlation of HR-HPV infection and p16 gene expression in cervical cancer

Objective To detect the expression of p16 and discuss the significance in genesis and development of cervical cancer. Methods FQ-PCR method was used to detect the expression levels of p16 mRNA in cervical cancer（CC）,cervical intraepithelial neoplasia（CIN）,and normal tissues.And in situ hybridization was used to detect HR-HPV in these tissues.Results The expression levels of p16 were 0.79±0.34,0.70±0.36,0.26±0.21 in CC,CIN and normal cervical tissues,respectively.The difference was significant （P〈0.05）.The expression level of p16 was significantly associated with FIGO stage （P〈0.05）,but it showed no significant relationship with age,tumor size,cell differential degree and lymphoid node metastasis （P〉0.05）.The expression level of p16 in HR-HPV positive cases was significantly higher than that in HR-HPV negative cases （P〈0.05）.Conclusion p16 shows high expression level in cervical cancer,which is closely related to the infection of HR-HPV infection.It suggests that the high expression of p16 play an important role in genesis and development of cervical cancer.