急性小肠梗阻时肠壁病理学及二胺氧化酶活性的变化

目的 探讨急性完全性小肠梗阻时血清和肠液中二胺氧化酶（DAO）活性改变机制及意义。方法 观察家兔小肠末端结扎梗阻模型在不同时相梗阻肠段的病理学改变,同时测定血清和肠液中DAO的浓度。结果 肠结扎后12小时家兔的肠壁出现充血、水肿、肠粘膜细胞凋亡等损伤性改变,肠液和血清中DAO浓度明显升高,肠结扎后24小时其粘膜部分坏死,DAO浓度继续升高,肠结扎48小时肠壁大部分坏死,肠液和血清中DAO浓度下降至肠结扎前水平。结论测定肠液和血清中DAO浓度的变化可能是诊断急性肠梗阻和选择手术时机的重要依据。

Diamine oxidase activty and pathological changes of intestine in acute intestinal obstruction

Objective To invstigate alterations of serum and intestinal juice diamine oxidase(DAO) activity in acute complete intestinal obstruction and its significance. Methods Terminal small intestine of rabbits was ligated. Then at different times afterwards pathological changes of the intestine were observed and concentration of DAO in serum and intestinal fluid were determined. Results 12 hours after the intestinal ligation, hyperemia, edema and apoptosis of intestinal mucosa were found with light and electron microscopy. The concentration of DAO in serum and intestinal juice increased markedly. After 24 hours of ligation, necrosis of in testinal mucosa was observed and concentraion of DAO increased more markedly, 48 hours afterwards, most part of intestine became necrotic, but the concentration of DAO returned to normal. Conclusions The determination of DAO in serum and in intestinal juice may be an important index for diagnosis of acute intestinal obstruction and for the choice of surgical management.