高血压脑梗死与血浆热休克蛋白70抗体水平关系的研究

目的通过测定高血压脑梗死患者血浆热休克蛋白70（HSP70）抗体水平，探讨HSP70抗体与脑梗死损伤程度的关系及其在脑梗死发病机制中的作用。方法100例脑梗死患者经头颅CT或MRI确诊，其中男64例，女36例；高血压者67例，血压正常者33例。选100名社区居民作为对照组，其中男58名，女42名；高血压者35名，血压正常者65名。采用蛋白质免疫印迹-酶联免疫吸附法（Western blot—ELISA）测定血浆HSP70抗体，并进行Logistic回归分析。结果脑梗死组HSP70抗体阳性率明显高于对照组（69％比13％）；脑梗死组高血压者HSP70抗体阳性率明显高于血压正常者（57％比12％），对照组高血压者HSP70抗体阳性率亦高于血压正常者（11％比2％）。上述结果差异均有显著性（P均〈0．05）。结论HSP70抗体增多与高血压脑梗死发病相关，可作为高血压脑缺血时一种分子生物学诊断指标；HSP70抗体表达越强，脑梗死病情越严重。因此，HSP70抗体增高是反映脑组织损伤程度的标志。

Study on plasma antibodies against heat shock protein 70 in the hypertensive patients with cerebral infarction

Objective To determine the level of heat shock protein 70 (HSP70) antibody in the hypertensive patients with cerebral infarction and explore the role of plasma antibody against HSP70 in the pathogenesis of cerebral infarction and its relationship to the brain tissue damage. Methods One hundred patients with CT or MRI confirmed cerebral infarction were enrolled in the study, in whom 64 were male, and 36 female. Among them, 67 patients were having hypertension and 33 with normal blood pressure. One hundred healthy individuals, including 58 male and 42 female, served as the control group. The level of plasma HSP70 antibody was determined with Western blot-enzyme linked immunoadsorbent assay (ELISA) technique. Results Patients with cerebral infarction had a significantly higher incidence (69%) of antibodies to HSP70 compared to the low incidence (13%) of antibodies to HSP70 in the controls. Patients with hypertension had a high incidence (57%) of antibodies to HSP70 compared to the low incidence (12%) of antibodies to HSP70 in patients with normal blood pressure in cerebral infarction group. Patients with hypertension in the control group had a higher incidence (11%) of antibodies to HSP70 compared to the low incidence (2%) of antibodies to HSP70 in the same group. Conclusion There is a significant difference in the level of antibodies to HSP70 between patients with cerebral infarction and controls. Elevation in the level of antibodies to HSP70 may reflect the degree of brain tissue damage.