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| 高效液相色谱法测定土霉素片的含量 | |
| 引用本文： | 乔晓芳. 高效液相色谱法测定土霉素片的含量[J]. 中国医药指南, 2014, 0(21): 80-81 |
| 作者姓名： | 乔晓芳 |
| 作者单位： | 河南省安阳市食品药品检验所,河南安阳455000 |
| 摘 要： | 目的建立土霉素片的含量测定方法。方法高效液相色谱法。采用Luna-C18色谱柱,以0.05 mol/L草酸铵溶液-二甲基甲酰胺-0.2 mol/L磷酸氢二铵(70∶25∶5)为流动相,用紫外检测器在282 nm波长处检测。结果土霉素片浓度在0.011 mg/mL范围内与峰面积线性关系良好(r=0.9990),平均回收率为100.1%。结论高效液相色谱法快速、简便、准确,可用于土霉素片的含量测定。 |
| 关 键 词： | 土霉素片 含量测定 高效液相色谱法 抗生素微生物检定法 |
Determination of the Content of Oxytetracycline Tablets by HPLC |
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| QIAO Xiao-fang. Determination of the Content of Oxytetracycline Tablets by HPLC[J]. Guide of China Medicine, 2014, 0(21): 80-81 | |
| Authors: | QIAO Xiao-fang |
| Affiliation: | QIAO Xiao-fang (Anyang Institute for Food and Drug Control, Anyang 455000, China) |
| Abstract: | Objective To establish a method for determination of the content of Oxytetracycline tablets. Methods High performance liquid chromatography. The WH-C18 column, with 0.05 mol/L ammonium oxalate solution-two methyl formamide-0.2 mol/L diammonium hydrogen phosphate （70∶25∶5）as mobile phase, using a UV detector at 282 nm wavelength. Results Oxytetracycline Tablets concentration relationship in 0.01-1 mg/mL scope with the peak area of linear（r=0.9990）, the average recovery rate was 100.1%. Conclusion The HPLC method is rapid, simple and accurate, can be used for the content determination of Oxytetracycline tablets. |
| Keywords: | Oxytetracycline tablets Content determination HPLC Microbiological method |
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