HSP70在喉乳头状瘤和声带白斑组织中的表达及其临床意义

目的: 探讨热休克蛋白70(HSP70)及其上游调控因子HSF1在喉乳头状瘤和声带白斑中的表达,阐明其在喉癌发生中的作用。方法:应用免疫组织化学法检测6例声带白斑 (声带白斑组)、6例喉乳头状瘤（喉乳头状瘤组）和7例声带息肉（对照组）的组织标本中HSP70和HSF1蛋白的表达。结果： HSP70和HSF1蛋白在喉乳头状瘤、声带白斑中的表达高于声带息肉,差异有统计学意义（P<0.05或P<0.01）；声带白斑在所有组织中表达最高。对声带息肉、喉乳头状瘤、声带白斑中HSP70蛋白表达量与HSF1表达的关系进行直线回归分析，结果显示，HSP70蛋白表达量与HSF1的变化呈现正的直线相关（r=0.867，P<0.01）。结论：HSP70和HSF1在声带白斑中的高表达可能与喉癌的发生密切相关，且HSP70和HSF1可能成为喉癌早期诊断的指标。

Expressions of HSP70 in laryngeal papilloma and vocal leukoplakia tissues and their clinical significances

Objective To study the expressions of heat shock protein 70(HSP 70) and its upstream regulation factor HSF1 in laryngeal papilloma and vocal leukoplakia and clarify their action in the genesis of human laryngeal carcinoma.Methods The expressions of HSP70 and HSF1 proteins were detected by immunohistochemical method in 6 specimens with vocal leukoplakia(vocal leukoplakia group),6 specimens with laryngeal papilloma(laryngeal papilloma group) and 7 specimens of vocal cord polyp(control group).Results The expressions of HSP70 and HSF1 in laryngeal papilloma and vocal leukoplakia were higher than that in vocal cord polyp,the differences were significant(P<0.05 or P<0.01),the expressions of HSP and HSF1 in vocal leukoplakia were the highest.Linear regression analysis of HSP70 protein and HSF1 expressions in vocal cord polyp,laryngeal papilloma and vocal leukoplakia showed positive linear correlation(r=0.867,P<0.01).Conclusion The overexpression of HSP70 and HSF1 protein in vocal leukoplakia may contribute to the genesis of human laryngeal carcinoma.HSP70 and HSF1 may be used as indicators for early diagnosis of laryngeal carcinoma.