鸦葱中黄酮成分的提取分离及结构鉴定

目的：提取、分离鸦葱中的黄酮成分并鉴定其结构，为开发利用其药用价值提供实验依据。方法：取鸦葱全草粉末，用70％乙醇提取，提取液经大孔树脂、硅胶、ODS柱、高效液相色谱仪分离纯化,并对分离的化合物进行结构鉴定。结果：分离得到3个化合物，经光谱学数据分析和理化常数鉴定其分别为：5,7,4′- 三羟基黄酮-8-C-β-D-吡喃葡萄糖碳苷、5,7,4′- 三羟基黄酮-6-C-α-L-吡喃阿拉伯糖8-C-β-D-吡喃葡萄糖碳苷及5,7,3′,4′- 四羟基黄酮-6-C-α-L-吡喃阿拉伯糖8-C-β-D-吡喃葡萄糖碳苷。结论：3种化合物均为首次从鸦葱中得到的黄酮苷类化合物。

Isolation and structure identification of flavonoid glycosides from Scorzonera austriaca Willd

Objective To isolate the flavonoid glycosides from Scorzonera austriaca Willd and identify its structure and to provide experimental evidences for medicinal values of flavonoid glycosides from Scorzonera austriaca Willd.Methods The herbs of Scorzonera austriaca Willd was extracted with 70% ethanol,the extractives were purified with macroporous resin,silica gel,ODS column chromatography and HPLC and the structure of componuds were identified.Results Three compounds were isolated,and their structures were identifited through spectroscopic analysis and physico-chemical constants.They were 5,7,4′-trihydroxy-flavone-8-C-β-D-glucopyranoside,5,7,4′-trihydroxy-flavone-6-C-α-L-arabinosyl-8-C-β-D-glucopyranoside and 5,7,3′,4′-tetrahydroxy-flavone-6-C-α-L-arabinosyl-8-C-β-D-glucopyranoside.Conclusion Three compounds are all regarded as flavonoid glycosides isolated from Scorzonera austriaca Willd for the first time.