萝卜硫素减轻心脏移植模型大鼠缺血/再灌注损伤

目的研究萝卜硫素在大鼠心脏移植缺血/再灌注损伤(I/RI)中的作用及PI3K/AKT/GSK-3β通路是否参与其中的作用。方法构建大鼠同种异体活体心脏移植模型为对照组,尾静注射萝卜硫素为萝卜磁素处理组(2.5 mg/kg)。比较大鼠的存活率、血清心肌酶、心肌细胞凋亡水平及心肌线粒体的肿胀程度;Western blot检测凋亡相关分子caspase-3、Bax和PI3K/AKT/GSK-3β的表达。结果萝卜硫素处理组的生存率显著高于对照组(P<0.05);萝卜硫素处理组不同处理时间(6、12、24及48 h)血清乳酸脱氢酶(LDH)、肌酸肌酶(CK)、肌酸肌酶同工酶(CK-MB)和肌钙蛋白T(TnT)的浓度均呈时间依赖性低于对照组。Caspase-3和Bax的表达明显降低(P<0.01),线粒体肿胀度也较对照组有所改善。结论萝卜硫素可能通过激活PI3K/Akt/GSK-3β通路抑制心肌细胞凋亡,改善大鼠心脏移植术后的I/RI。

Sulforaphane alleviates ischemia-reperfusion injury of heart transplantation in rat models

Objective To investigate the effect of sulforaphane on ischemia-reperfusion injury(I/RI)and the role of PI3K/Akt/GSK-3βpathway in heart transplantation of rats.Methods The rat model of allograft heart transplantation was established as the control group.The survival rate,the level of serum myocardial enzymes,apoptosis of myocardial cells and the swelling degree of myocardial mitochondria were compared with sulforaphane injection group(2.5 mg/kg).Western blot was used to detect the expression of apoptotic molecules caspase-3,Bax and PI3K/Akt/GSK-3β.Results The survival rate of sulforaphane group was significantly higher than that of control group(P<0.05).The serum concentrations of LDH,CK,CK-MB and TNT in sulforaphane group were lower than those in control group with a time dependent manner at different treatment time(6,12,24 and 48 h).The expressions of caspase-3 and Bax were significantly decreased(P<0.01),and the degree of mitochondrial swelling was also improved compared with the control group.Conclusions Sulforaphane may inhibit myocardial apoptosis by activating PI3K/Akt/GSK-3βpathway and improve I/RI after heart transplantation as shown by animal model.