20(S)-人参皂苷Rg3抑制人急性单核细胞白血病THP-1细胞迁移和侵袭

目的：观察低极性20(S)-人参皂苷Rg₃(S-Rg₃)体外抑制人急性单核细胞白血病THP-1细胞迁移和侵袭的作用。方法：将不同浓度(0、20、40和80 mg/L)的S-Rg₃加入THP-1细胞培养体系，Transwell小室检测S-Rg₃抑制细胞迁移的作用。采用免疫细胞化学、免疫荧光、Western blot和RT-qPCR等多种方法，检测S-Rg₃对THP1细胞黏附、迁移和侵袭相关蛋白表达的影响及其相关机制。结果：S-Rg₃能有效地抑制THP-1细胞迁移，下调黏附相关蛋白N-cadherin，迁移相关蛋白C-X-C趋化因子受体4(CXCR4)和基质细胞衍生因子1(SDF-1)，以及侵袭相关蛋白基质金属蛋白酶2(MMP2)的表达，但上调抑制迁移侵袭相关蛋白E-cadherin、金属蛋白酶组织抑制物1(TIMP1)和TIMP3表达，同时下调CXCR4 mRNA表达，上调E-cadherin和TIMP1 mRNA表达，呈剂量依赖性。此外，S-Rg_...

Inhibitory effect of 20(S)-ginsenoside Rg;on migration and invasion of human acute monocytic leukemia THP-1 cells

AIM:To observe the effect of low-polarity 20(S)-ginsenoside Rg;(S-Rg;)on the migration and invasion of human acute monocytic leukemia THP-1 cells.METHODS:The THP-1 cells were treated with various concentrations(0,20,40 and 80 mg/L)of S-Rg;. The cell migration was detected by Transwell assay. Immunocytochemistry,immunofluorescence,Western blot and RT-qPCR were used to detect the expression of various proteins related to adhesion,migration,invasion and the related mechanisms.RESULTS:Treatment with S-Rg;inhibited the migration of THP-1 cells,and down-regulated the expression of adhesion-related protein N-cadherin,migration-related proteins C-X-C chemokine receptor 4(CXCR4)and stromal cell-derived factor-1(SDF-1),and invasion-related protein matrix metalloproteinase 2(MMP2),but up-regulated the expression of E-cadherin,tissue inhibitor of metalloproteinase 1(TIMP1)and TIMP3. It also decreased CXCR4 mRNA expression,and stimulated E-cadherin and TIMP1 mRNA expression in a dosedependent manner. In addition,S-Rg;inhibited PI3 K/AKT signaling pathway.CONCLUSION:Treatment with S-Rg;effectively inhibits the migration and invasion of THP-1 cells,which may be related to inhibition of PI3 K/AKT signaling pathway.