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| 敲除EZH2抑制人宫颈癌细胞系迁移和侵袭 | |
| 引用本文： | 陈茜,刘宪,崔南,翟阳. 敲除EZH2抑制人宫颈癌细胞系迁移和侵袭[J]. 基础医学与临床, 2022, 42(3): 395-400. DOI: 10.3969/j.issn.1001-6325.2022.03.008 |
| 作者姓名： | 陈茜 刘宪 崔南 翟阳 |
| 作者单位： | 西安交通大学第一附属医院 生殖医学科,陕西 西安710061,陕西省肿瘤医院 肿瘤内科,陕西 西安710061 |
| 基金项目： | 国家自然科学基金(81672910)； |
| 摘 要： | 目的 研究zeste基因同源蛋白2(EZH2)对人宫颈癌细胞系迁移和侵袭的影响,并初步探讨其作用机制.方法 利用CRISPR/Cas9敲除人宫颈癌HeLa和人子宫颈鳞癌SiHa细胞系中EZH2的表达.划痕实验检测细胞迁移;Transwell小室法检测细胞体外迁移和侵袭;实时荧光定量PCR检测STAT3 mRNA水平;W... |
| 关 键 词： | 宫颈癌 EZH2 迁移和侵袭 STAT3信号通路 |
Knockout of EZH2 inhibits migration and invasion of human cervical cancer cell lines |
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| CHEN Qian,LIU Xian,CUI Nan,ZHAI Yang. Knockout of EZH2 inhibits migration and invasion of human cervical cancer cell lines[J]. Basic Medical Sciences and Clinics, 2022, 42(3): 395-400. DOI: 10.3969/j.issn.1001-6325.2022.03.008 | |
| Authors: | CHEN Qian LIU Xian CUI Nan ZHAI Yang |
| Affiliation: | (Department of Reproductive Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061;Department of Oncology, Tumor Hospital of Shaanxi Province, Xi'an 710061, China) |
| Abstract: | Objective To explore the effect of EZH2 on the migration and invasion of human cervical cancer cell lines and its mechanism.Methods CRISPR/Cas9 was used to knockout EZH2 expression in HeLa and SiHa cell lines.The migration was detected by scratch test,and the migration and invasion in vitro was detected by Transwell migration and invasion test.The STAT3 mRNA level was detected by real-time fluorescence quantitative PCR,and the expression of STAT3 and p-STAT3 proteins was detected by Western blot.ResultsCRISPR/Cas9 could knockout EZH2 expression.The wound healing rate of HeLa and SiHa cells in EZH2 knockout group at 24 h and 48 h,and the counting of transmembrane cell migration and invasion in Transwell chamber were lower than those in control group(P<0.05).Compared with the control group,the protein level of p-STAT3 in EZH2 knockout group was significantly decreased(P<0.05).Conclusions EZH2 plays an important role in promoting the migration and invasion of cervical cancer cells,which may be related to the targeted regulation of STAT3 pathway,and is a potential target in the treatment of cervical cancer. |
| Keywords: | cervical cancer EZH2 migration and invasion STAT3 signaling pathway |
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