电子自旋共振波谱法检测辐照中成药颗粒制剂

目的利用60Co-γ射线辐照对电子自旋共振（ESR）波谱特性的影响,建立简便、快捷的辐照中药颗粒制剂检测方法。方法 2种中成药颗粒（妇乐颗粒和抗感解毒颗粒）样品分别经剂量率为1.0kGy/h,剂量为0、0.1、0.3、0.7、1、3、5、7、9kGy的60Co-γ射线辐照处理,用顺磁共振波谱检测仪对样品ESR波谱特性进行检测,样品量为0.05g,每个样品3次重复。结果辐照前后ESR波谱峰值有明显差异,ESR信号强度随辐照样品吸收剂量的增加而增强;样品在室温下保存120d,ESR信号强度随时间虽有变化,但仍处于与对照组呈显著差异的水平。结论利用ESR波谱法可简便、快捷、有效地鉴别中药颗粒制剂是否经辐照处理。

Detection of irradiated granules preparation of Chinese patent medicine by ESR spectroscopy

Objective To develop a simple and convenient method to detect the granules preparation of Chinese patent medicine （GPCPM）, based on the effect of ESR spectroscopy using 60Co-γ ray irradiation. Methods Two kinds of GPCPM were irradiated by 60Co-γ ray under nine doses （0, 0.1, 0.3, 0.7, 1, 3, 5, 7, and 9 kGy） at 1.0 kGy/h dose rate. The ESR spectroscopy was characterized by ESR detector with 0.05 g of sample and repeated for three times. Results It was shown that the ESR spectra were obviously different with or without irradiation. The intensity of ESR signal increased with the irradiation dose increasing, but decreased with the storage time expansion with a linear correlation. The intensity of ESR was weakened gradually during the storage at the room temperature for 120 d, though the ESR signal could be still detected. Conclusion The method of ESR spectroscopy is simple, rapid, and practically valuable for the identification of GPCPM irradiated or not.