尘肺尸检主动脉组织中脂类含量的测定

目的建立经甲醛固定的主动脉组织中脂类测定方法，同时观察煤工尘肺尸检主动脉组织中脂类变化规律是否与肺纤维化有关。方法用改进FoIch法制备样本，在康宁1200E型生化自动分析仪上测定甘油三酯及胆固醇。结果用Folch法测定甲醛固定后的主动脉组织中脂类（GT，CH）含量是可行的。随着全肺肺组织纤维化程度的进展，年龄的增加，主动脉组织中的甘油三酯含量逐渐恢复到正常人的水平；胆固醇的变化与甘油三酯相反。结论矽肺民肺组织中饱和磷脂和甘油磷脂增加，接尘工人主动脉组织中也不例外，煤工生肺尸检主动脉组织中脂类变化规律与肺纤维化有关。

DETERMINATION OF ADIPOSE CONTENT IN AORTA TLSSUE FROM PNEUMOCONIOSIS

Objective:To set up method of determinig adipose content in aorta tissne from pneumoconiosisfixed with formaldehyde,and to study the relationship between the change law of the adipose content in anrta tissueand lung fibrinous in the patients with pneumoconiosis. Methods: Prepared with improved Folch method, the samgleswere used to datermine the contents of triglyceride and cholesterol with the Kangning-1200E Biochemistry Automatictissue from pneunmoconiosis fixed with formaldehyde with improved Folch method Following the development of thelung fibrinous in the patients with pneumoconiosis, the contents of triglyceride in the aorta tissue form pneumoconiosisdevelopent of the lung fibrinous in the patients with pneumoconiosis, the contents of triglyceride in the aorta tissuerecovered gradually to normal level. The change of cholesterol was oppeite to the change of triglyceride. Conclusion:The change law of the adipose content in ptomatopsia aorta tissue is related to lung fibrinous in the patients with pneumoconuosis