壳聚糖-磷酸化壳聚糖聚电解质复合物水凝胶的组装与仿生矿化

摘要 背景：依据生物矿化有机基质模版调控无机晶体生长理论，采用分子仿生技术构建人体硬组织修复材料是目前生物材料研究的热点之一。但是目前还未见构建聚电解质复合体有机基质模板进行骨组织修复材料仿生合成的报道。 目的：以聚阳离子壳聚糖与其聚阴离子衍生物磷酸化壳聚糖形成的聚电解质复合物水凝胶作为生物矿化有机基质模板的预组织形式，进行生物材料仿生合成。 方法：首先对天然聚阳离子多糖壳聚糖进行磷酸化改性，合成聚阴离子的磷酸化壳聚糖；然后通过聚阳离子壳聚糖溶液与聚阴离子磷酸化壳聚糖溶液复合，形成聚电解质复合物水凝胶；最后将获得的聚电解质复合物水凝胶浸入钙磷过饱和液中，诱导磷灰石晶体在水凝胶基质的调控下沉积生长。通过傅里叶红外光谱、X射线衍射、扫描电镜进行表征观察。 结果与结论：阳离子壳聚糖与聚阴离子磷酸化壳聚糖相互作用，形成多孔的聚电解质复合物水凝胶，此水凝胶可以诱导磷灰石晶体的生长。从而仿生合成了一种新型的有机无机复合生物材料。 关键词：壳聚糖；磷酸化壳聚糖；聚电解质复合物；水凝胶；仿生矿化 doi:10.3969/j.issn.1673-8225.2010.47.018

Assemble and biomimetic mineralization of chitosan-phosphorylated chitosan polyelectrolyte complex hydrogel

Abstract BACKGROUND: Based on the theory of biomineralized organic matrix template controls inorganic crystal growth, human hard tissue repair materials constructed using molecular bionic technology is one of the research hotspots of biological materials. But there is no report regarding polyelectrolyte complex organic matrix plate constructed for biomimetic synthesis of bone tissue repair materials. OBJECTIVE: Polyelectrolyte complex hydrogel formed by reacting polycation chitosan and polyanion derivate phosphorylated chitosan is used as the pre-tissue biomineralized organic matrix template to biomimetic synthesis of the biomaterials. METHODS: Firstly natural polycation polysaccharide chitosan was modified through phosphorylation, to synthesize polyanion derivate phosphorylated chitosan; then polyelectrolyte complex hydrogel was formed from equal volumes of phosphorylated chitosan solution and polycation chitosan solution. The obtained polyelectrolyte complex hydrogel was soaked in supersaturated CA/P solution to induce hydroxyapatite crystals deposit and grow under the regulation of hydrogel matri. It was characterized with fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscope. RESULTS AND CONCLUSION: The porous polyelectrolyte complex hydrogel formed by the interaction between polycation chitosan and polyanion phosphorylated chitosan can induce hydroxyapatite crystals nucleated and grew on the fiber surfaces of the hydrogel, thus biomimetic synthesizing a new composite biomaterial for bone repairing.