| 危重症手足口病患儿淋巴细胞亚群的检测 |
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| 引用本文： | 莫扬,李智山.危重症手足口病患儿淋巴细胞亚群的检测[J].中国实验诊断学,2011,15(12):2044-2045. |
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| 作者姓名： | 莫扬 李智山 |
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| 作者单位： | 襄阳市中心医院,检验科,河北,襄阳441021 |
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| 摘 要： | 目的研究危重症手足口病患儿外周血淋巴细胞亚群的变化,以探讨其免疫功能改变。方法以临床确诊并入住ICU的40例危重症手足口病患儿为研究对象,并与30例正常儿童进行比对研究,应用流式细胞仪检测外周抗凝全血的淋巴细胞亚群：T淋巴细胞（CD3＋CD19-）、辅助T细胞Th（CD3＋CD4＋）、抑制T细胞Ts（CD3＋CD8＋）、B细胞（CD3-CD19＋）和NK细胞（CD3-CD56＋）的相对计数,并计算Th/Ts值。结果危重症手足口病患儿外周血T淋巴细胞、Th细胞、Ts细胞的百分率下降,均明显低于对照组（P〈0.05）;B细胞的百分率则显著增高（P〈0.05）;而两组间NK细胞百分率及Th/Ts细胞比值的变化不存在明显差异（P〉0.05）。结论危重症手足口病患儿出现细胞免疫及体液免疫功能紊乱。
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| 关 键 词： | 手足口病 淋巴细胞亚群 流式细胞术 |
Analysis on peripheral blood lymphocyte subsets in children with critical hand-foot-mouse disease |
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| MO Yang,LI Zhi-shan.Analysis on peripheral blood lymphocyte subsets in children with critical hand-foot-mouse disease[J].Chinese Journal of Laboratory Diagnosis,2011,15(12):2044-2045. |
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| Authors: | MO Yang LI Zhi-shan |
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| Institution: | MO Yang,LI Zhi-shan.(Department of Laboratory Medicine,Xiangyang central hospital,Hubei 441021,China) |
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| Abstract: | Objective To study the variation of peripheral blood lymphocyte subsets and immune function in critical hand-foot-mouse disease(HFMD) children.Methods Forty cases with critical HFMD and thirty healthy children as controls were enrolled in the study.The percentage of lymphocyte subsets,including CD3+CD19-cells(T),CD3+CD4+cells(Th),CD3+CD8+cells(Ts),CD3-CD19+cells(B),CD3-CD56+cells(NK),in anticoagulative whole blood of patients from the two groups were determined by flow cytometry.Results Compared with normal... |
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| Keywords: | hand-foot-mouth disease lymphocyte subsets flow cytometry |
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