| Detection of thiopurine s-methyltransferase mutations by template directed determinator in incorporation with fluorescence polarization （TDI-FP） |
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| 引用本文： | 白玉杰,赵锦荣,张文红,薛丽,郭晏海,闫小君. Detection of thiopurine s-methyltransferase mutations by template directed determinator in incorporation with fluorescence polarization （TDI-FP）[J]. 中国人民解放军军医大学学报, 2003, 18(6): F002-F002,392,393 |
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| 作者姓名： | 白玉杰 赵锦荣 张文红 薛丽 郭晏海 闫小君 |
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| 作者单位： | InstituteofGeneDiagnosticTechnology,FourthMilitaryMedicalUniversity,Xi‘an710033,China |
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| 基金项目： | ShaanxiScience&TechnologyFoundation(No .2 0 0 3C2 0 3) |
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| 摘 要： | Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT^*1,^*3A,^*3B and ^*3C in a healthy Chinese population. Methods:A TDI-FP assay system was set up in out lab. To evaluate this system, 220 healthy individuals were analyzed for the polymorphic sites at positions 460 (G→A)and 719 (A→G)of the TPMP gene using our new TDI FP method. Results:Three TPMP *3C(G^460→G^719) heterozygotes were identified, TPMP ^*3A and TPMP ^*3B were not found. All mutations were confirmed by conventional DNA sequencing analysis. Conclusion:TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping. TPMP ^*3C was the only polymorphism identified in this clinical samples we have registered.
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| 关 键 词： | s-转甲基酶 模板定量化学测定仪 TDI-FP TPMT基因 基因转换 |
Detection of thiopurine s-methyltransferase mutations by template directed determinator in incorporation with fluorescence polarization(TDI-FP) |
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| BAI Yu-jie,ZHAO Jin-rong,ZHANG Wen-hong,XUE Li GUO Yan-hai,YAN Xiao-jun Institute of Gene Diagnostic Technology,Fourth Military Medical University,Xi'an ,China. Detection of thiopurine s-methyltransferase mutations by template directed determinator in incorporation with fluorescence polarization(TDI-FP)[J]. Journal of Medical Colleges of PLA(China), 2003, 18(6): F002-F002,392,393 |
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| Authors: | BAI Yu-jie ZHAO Jin-rong ZHANG Wen-hong XUE Li GUO Yan-hai YAN Xiao-jun Institute of Gene Diagnostic Technology Fourth Military Medical University Xi'an China |
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| Affiliation: | BAI Yu-jie,ZHAO Jin-rong,ZHANG Wen-hong,XUE Li GUO Yan-hai,YAN Xiao-jun Institute of Gene Diagnostic Technology,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | Objective:To develop a new method for the detection of TPMT gene mutations and determine thefrequencies of four TPM alleles,TPMT*1,*3A,*3B and *3C in a healthy Chinese population.Methods:A TDI-FP assay system was set up in out lab.To evaluate this system,220 healthy individuals were analyzed for the polymorphisites at positions 460(G→A)and 719(A→G)of the TPMP gene using our new TDI-FP method.Results:Three TPMP*3C(G460→G719)heterozygotes were identified,TPMP *3A and TPMP *3B were not found.All mutations were confirmed by conventional DNA sequencing analysis.Conclusion:TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping.TPMP *3C was the only polymorphism identified in this clinical samples we have registered. |
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| Keywords: | thiopurine S-methyltransferase (TPMT) template- directed day-terminator incorporation (TDI) fluorescence polarization (FP) genotype polymorphism |
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