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Oligonucleotide mapping of picornavirus RNAs by two-dimensional electrophoresis.
Authors:D P Frisby  C Newton  N H Carey  P Fellner  J F Newman  T J Harris  F Brown
Affiliation:Searle Research Laboratories, High Wycombe, Buckinghamshire, England;Animal Virus Research Institute, Pirbright, Surrey, England
Abstract:
Considerable differences have been found in two-dimensional polyacrylamide gel electrophoresis fingerprints of complete ribonuclease T1 digestion products of the RNAs of representative members of the entero-, cardio-, and foot-and-mouth disease virus subgroups of the picornavirus family. Individual members of the different subgroups, serotypes of a virus, and even subtypes within a serotype can be distinguished by the use of this technique. The method has also facilitated the identification of homopolymeric regions within the different picornavirus genomes, and the presence of a poly(C) tract in the cardio- and foot-and-mouth disease virus subgroups has been confirmed. A poly(A)-rich tract of approx 40–100 nucleotides has been detected in all the picornaviruses studied. Oligonucleotide fragments possibly specific to the enterovirus subgroup were also detected and partially characterised.
Keywords:
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