河南地区HIV-1毒株Gag基因抗原表位变异特征及准种特点研究

目的 探讨中国河南地区人免疫缺陷病毒Ⅰ型(HIV-1)毒株GAG蛋白抗原表位变异特征,并对其准种特点加以分析.方法 套式聚合酶链反应(Nested-PCR)扩增确认HIV阳性样本gagp17～p24基因区段并测序,PCR产物纯化后克隆,挑选克隆株鉴定为阳性后测序,以MEGA(version 3.0)等软件进行分析.结果 河南HIV毒株为B'亚型;gag基因p17区段抗原表位突变有E62G(55.80%),Y79F(48.90%),T84V(48.90%),144V(44.20%),gag基因p24区段抗原表位未见明显变异.结论 HIV-1 B'亚型毒株gag基因p17区段的4个抗原表位,存在较大变异,p24区段较为保守,适合抗原表位疫苗的研制.

Study of Gag gene antigen epitypes variation and the quasispecies group characteristics in Henan area HIV-1 strains

Objective To study the characteristics of the variation of antigen epitopes and quasispecies group in the HIV-1 viruses in Henan province in China. Methods The region of the p17-p24 of the HIV-1 gag gene was amplified by nested polymerse chain resction(PCR), purified products were cloned into the vector, the obtained were analyzed by MEGA soft wares. Results B' subtype strains were predominant in Henan province, the mutations in antigen epitypes of the p17 region of the gag gene focus on E62g(55.8% ) ,Y79f(48.9% ) ,T84V (48.9), I44V(44.2%), the p24 region had not found the distinct mutation. Conclusion Both the PCR sequences and clone strains sequences demonstrated that four antigen epitopes mutations in p17 region of the HIV B' subtype gag gene, and the region of p24 was more conservative,which was suitable for development of the epitope vaccien.