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| 姜黄素对涎腺腺样囊性癌细胞系SACC-83增殖和凋亡的影响 | |
| 引用本文： | 杨威,陈彪,张英怀,贾志宇,屈鹏飞,郭利华,张平,唐全勇. 姜黄素对涎腺腺样囊性癌细胞系SACC-83增殖和凋亡的影响[J]. 现代口腔医学杂志, 2009, 23(2): 142-144 |
| 作者姓名： | 杨威 陈彪 张英怀 贾志宇 屈鹏飞 郭利华 张平 唐全勇 |
| 作者单位： | 河北医科大学第二医院口腔颌面外科,石家庄,050000 |
| 基金项目： | 河北省科技攻关指导项目 |
| 摘 要： | 目的体外研究姜黄素对涎腺腺样囊性癌细胞系SACC-83增殖和凋亡的影响。方法用终浓度为2.5、5、10、15、20mg/L的姜黄素处理SACC-83。应用MTT比色试验,倒置显微镜,Giemsa染色,Annexin-V-FITC和PI双标记活细胞后流式细胞仪和透射电镜检测和观察SACC-83的生长抑制率和细胞凋亡情况。结果姜黄素对SACC-83的生长抑制率与药物浓度和作用时间具有明显的正相关性（P〈0.01）。用药组细胞明显凋亡,凋亡率随处理时间延长和药物浓度增加而上升,各组间有显著差异（P〈0.01）。结论姜黄素具有抑制涎腺腺样囊性癌细胞系SACC-83增殖和诱导其凋亡的作用,且具有浓度和时间依赖性。 |
| 关 键 词： | 姜黄素 腺样囊性癌 涎腺 增殖 凋亡 |
Effect of curcumin on proliferation and apoptosis of salivary adenoid cystic carcinoma cell line SACC-83 in vitro |
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| Affiliation: | YANG Wei, CHEN Biao, ZHANG Yinghuai, et al. (Department of Oral and Maxillofacial Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000) |
| Abstract: | Objective To evaluate effects of curcumin on proliferation and apoptosis of salivary adenoid cystic carcinoma cell line SACC - 83 in vitro. Methods SACC - 83 cells were treated with different concentration of curcumin （2.5, 5, 10, 15, and 20mg/L respectively）. MTr assay, Giemsa staining, flow cytometry with Annexin- V- FITC/ propidiumiodide （PI） double staining, and transmission electron microscope （TEM） were used to evaluate cell growth and apoptosis. Results The growth inhibition rate of curcumin on SACC - 83 cells was positively correlated with concentrations of curcumin and treated time. The apoptosis of SACC - 83 cells was increased with concentration and treatment time of curcumin. Conclusion Curcumin could effectively inhibit proliferation and induce apoptosis of salivary gland adenoid cystic carcinoma cell line SACC -83. |
| Keywords: | Curcumin Adenoid cystic carcinoma Salivary gland Proliferation Apoptosis |
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