Fluorescence in situ hybridization BCR/ABL fusion signal rate in interphase nuclei of healthy volunteer donors: a test study for establishing false positive rate |
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Authors: | Kowalczyk Jerzy R Gaworczyk Anna Winnicka Dorota Lejman Monika Babicz Mariusz |
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Institution: | Department of Pediatric Hematology and Oncology, Cytogenetic Laboratory, Children's University Hospital, Chodzki Strasse 2, 20-093 Lublin, Poland. |
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Abstract: | Fluorescence in situ hybridization (FISH) using chromosome-specific DNA probes is rapidly becoming a part of clinical laboratory practice. However, as a relatively new clinical test, it is not yet standardized and for practical reasons each laboratory must establish its own criteria. For this purpose we have evaluated the specificity of a dual-color BCR/ABL translocation probe by establishing the range of BCR/ABL fusion-positive scores in a healthy donor group. The false positive rate (FPR), determined by the percent of FISH BCR/ABL fusion-positive cells found in the specimens of healthy donors, was estimated at 2.3% (mean = 1%-4%). Thus the cut-off value for false positive nuclei was set at 5%. |
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