| 响应面法优化女贞子中总三萜的提取工艺研究 |
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| 引用本文： | 吴修红,孙泽,杨恩龙,于丹,吴军凯. 响应面法优化女贞子中总三萜的提取工艺研究[J]. 上海中医药杂志, 2016, 50(1): 83-87 |
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| 作者姓名： | 吴修红 孙泽 杨恩龙 于丹 吴军凯 |
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| 作者单位： | 黑龙江中医药大学中医药研究院,黑龙江哈尔滨,150040,黑龙江中医药大学中医药研究院,黑龙江哈尔滨,150040,黑龙江中医药大学中医药研究院,黑龙江哈尔滨,150040,黑龙江中医药大学中医药研究院,黑龙江哈尔滨,150040,黑龙江中医药大学中医药研究院,黑龙江哈尔滨,150040 |
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| 基金项目： | 教育部博士点基金资助项目(20122327120005)；黑龙江省自然科学基金资助项目(LC2011C32)；黑龙江中医药大学学生创新基金资助项目 |
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| 摘 要： | 目的采用星点设计-响应面法优选女贞子中总三萜的乙醇回流提取工艺。方法在单因素考察基础上,利用三因素三水平响应面法研究乙醇浓度、提取时间、溶媒比对女贞子总三萜得率的影响。结果确定最优提取工艺为12倍量67%乙醇提取2次,每次1.70 h;女贞子总三萜提取量为57.62 mg/g,与预测值的偏差为2.89%。结论星点设计-响应面法优化的女贞子总三萜提取工艺方法简单,精密度高,可预测性较优。
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| 关 键 词： | 女贞子 总三萜 响应面法 乙醇回流提取法 |
| 收稿时间： | 2015-03-25 |
Optimization of extraction process for total triterpenoids in Ligustrum lucidum Ait. by response surface methodology |
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| WU Xiu-hong,SUN Ze,YANG En-long,YU Dan and WU Jun-kai. Optimization of extraction process for total triterpenoids in Ligustrum lucidum Ait. by response surface methodology[J]. Shanghai Journal of Traditional Chinese Medicine, 2016, 50(1): 83-87 |
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| Authors: | WU Xiu-hong SUN Ze YANG En-long YU Dan WU Jun-kai |
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| Affiliation: | Academy of Traditional Chinese Medicine and Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040,Academy of Traditional Chinese Medicine and Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040,Academy of Traditional Chinese Medicine and Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040,Academy of Traditional Chinese Medicine and Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040 and Academy of Traditional Chinese Medicine and Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040 |
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| Abstract: | Objective:To optimize ethanol reflux extraction process of total triterpenoids in Ligustrum lucidum Ait. MethodsOn the basis of single factor tests,response surface analysis of three-factor-three-level was adopted to investigate effects of ethanol concentration,extracting time and solvent fold on yield of total triterpenoids. Results:Optimal extraction conditions were as follows:extracted 2 times by 12 times the count of 67% ethanol with 1.70 h each time; under these conditions,yield of total triterpenoids was 57.62 mg/g,2.89% in low deviation than predicted. Conclusion:This extraction process of total triterpenoids from L. Lucidum dahurica by central composite design/response surface methodology was a simple method with high precision and good predictability. |
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| Keywords: | Ligustrum lucidum Ait. total triterpenoids response surface methodology ethanol reflux extraction |
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