重组干扰素-γ对哮喘患者外周血单个核细胞IL-8和IL-10的作用研究

目的 研究重组干扰素－γ对哮喘患者体外单个核细胞ＩＬ－８和ＩＬ－１０释放的影响以及与血清ＥＣＰ水平的关系。方法 取哮喘患者８例、正常人５例的外周静脉血,分离单个核细胞培养,６６ｈ后将哮喘患者的ＰＢＭＣ上清液分为重组干扰素－γ干预组、氟美松干预组、未加干预物组,７２ｈ收集上清液测定ＩＬ－８和ＩＬ－１０的浓度。同时留取血清测ＥＣＰ。结果 哮喘者血清ＥＣＰ值与ＰＢＭＣ培养细胞上清液中ＩＬ－８值呈现显著正相关（ｒ＝０．７０１）,与ＩＬ－１０值呈显著负相关（ｒ＝０．６３８）。者喘患者血清中ＥＣＰ和ＰＢＭＣ上清液ＩＬ－８和ＩＬ－１０浓度与正常相比有显著性差异（Ｐ〈０．０５）。ｒＩＦＮ－γ干预后ＰＢＭＣ上清中ＩＬ－８较未干预组显著下降（Ｐ〈０．０５）,而ＩＬ－１０显著增高（Ｐ〈０．０５）;氟美松干预组则无明显差异性。结论 ｒ

Effect of recombinantinterferon-

Objective To study the effect of （rIFN-γ）on IL-8 and IL-10release in asthmatic patients PBMC and its relationship with serum level of ECP．Methods Samples of PBMC obtained from 8 cases of asthmatic patients and five cases of normal persons were cultured in RPMI 1640 containing 10％ bovine serum．After 66 hours,the supernatants of cultured PBMC of asthmatic patients were divided into three groups：group A （rIFN-γ）,group B （Dexamethasone）and group C （control group）．Concentration of IL-8 and IL-10 in the supernatant in the three groups was determined after 72 hours．Serum ECP levels were also determined．Results The serum ECP level was positively and signifocantly correlated with content of IL-8 in the supernatant （r＝0.701）and negatively correlated With that of IL-10（r＝0.638）．There was significant difference between the serum ECP level and the IL-8 and IL-10 concentration in supernatants of cultured PBMC （P＜0.05）．After rIFN-γ intervention ,the IL-8 level decreased significantly （P＜0.05）and the IL-10 increased singificantly（P＜0.05）than that of control group．There was no significant difference after dexamethasone intervention．Conclusion rIFN-γ may decrease the level of IL-8 which had anti-inflammation effects．IL-10 may inhibit the proliferation and differentiation of inflammatory cells caused by antigen stimulation and may inhibit the synthesis of cytokines and inflammatory mediators and also inhibit B cells to secret IgE．rIFN-γ can promote the synthesis and secretion of IL-10 which can increase its anti-inflammatory function．