| HPLC法测定乐脉颗粒中羟基红花黄色素A的含量 |
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| 引用本文： | 孟珺,付春梅,刘丽娟,乔瑞红.HPLC法测定乐脉颗粒中羟基红花黄色素A的含量[J].中国药事,2009,23(3):284-285. |
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| 作者姓名： | 孟珺 付春梅 刘丽娟 乔瑞红 |
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| 作者单位： | 1. 北京大学深圳医院,深圳,518036
2. 四川大学华西药学院 |
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| 摘 要： | 目的建立测定乐脉颗粒中羟基红花黄色素A含量的高效液相色谱法。方法采用迪马Diamonsil C18色谱柱,甲醇-0.1%磷酸（30∶70）为流动相,流速1.0mL·min^-1,检测波长403nm,柱温：35℃。结果羟基红花黄色素A在0.0053～0.106mg·mL^-1范围内线性关系良好（r=0.9998）。乐脉颗粒的平均加样回收率为99.5%,RSD=1.8%（n=9）。结论该方法简便快速,适用于乐脉颗粒中羟基红花黄色素A的含量测定。
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| 关 键 词： | HPLC 乐脉颗粒 羟基红花黄色素A |
Determination of Hydroxysafflor Yellow A in Lemai Granules by HPLC |
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| Meng Jun,Fu Chunmei,Liu Lijuan,Qiao Ruihong.Determination of Hydroxysafflor Yellow A in Lemai Granules by HPLC[J].Chinese Pharmaceutical Affairs,2009,23(3):284-285. |
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| Authors: | Meng Jun Fu Chunmei Liu Lijuan Qiao Ruihong |
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| Institution: | Meng Jun, Fu Chunmei, Liu Lijuan and Qiao Ruihong (Shenzhen Hospital Affiliated to Peking University, Shenzhen 518036 ; 1West China School of Pharmacy Sichuan University) |
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| Abstract: | Objective To develop a method for the determination of hydroxysamor yellow A in Lemai Granules by HPLC. Methods The column Dikma Diamonsil C18 （4.6mm× 150mm, 5μm） was used. The mobile phase was methanol 0. 1% phosphorid acid （30 ： 70） at flow rate of 1.0mL · min^-1 and the detection wavelength was set at 403nm. The column temperature was 35℃. Results The calibration curve was linear in the range of 0. 0053-0. 106mg · mL^-1 （r=0. 9998）, the average recovery was 99.5% with RSD= 1.8% （n = 9）. Concluions This method is simple, sensitive and suitable for the assay of hydroxysafflor yelow A in Lemai Granules. |
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| Keywords: | HPLC |
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