| miR-146a对小鼠巨噬细胞IL-18表达的影响 |
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| 引用本文： | 项洋,姜明红,王东生,高静,张亚玺,刘彦信,郑德先. miR-146a对小鼠巨噬细胞IL-18表达的影响[J]. 细胞与分子免疫学杂志, 2011, 27(5): 477-479 |
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| 作者姓名： | 项洋 姜明红 王东生 高静 张亚玺 刘彦信 郑德先 |
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| 作者单位： | 中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京,100005 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:探讨miR-146a对小鼠单核-巨噬细胞株RAW264.7以及小鼠原代腹腔巨噬细胞Thl/Th2类细胞因子表达的影响。方法:体外培养的小鼠单核-巨噬细胞系RAW264.7细胞和腹腔新鲜分离的巨噬细胞分别瞬时转染miR-146a mimics、阴性对照mimics(NC mimics)、抑制性miR-146a(miR-146a inhibitor)和抑制性miR-146a阴性对照(NC in-hibitor)。转染后,利用real time PCR定量检测IL-18、IL-5和IL-10的表达情况。结果:RAW264.7细胞和原代腹腔巨噬细胞在转染miR-146a mimics后IL-18的表达水平明显降低(P<0.05),转染miR-146a inhibitor后IL-18的表达水平明显升高(P<0.05),但对IL-5和IL-10的表达,两种转染形式均没有影响。结论:巨噬细胞RAW264.7及原代巨噬细胞表达的miR-146a能够负向调控Thl类细胞因子IL-18的表达,但是不能调控Th2类细胞因子IL-5及IL-10的表达。
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| 关 键 词： | 巨噬细胞 miR-146a Th1/Th2细胞因子表达 |
Effect of miR-146a on IL-18 expression in mouse macrophage |
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| XIANG Yang,JIANG Ming-hong,WANG Dong-sheng,GAO Jing,ZHANG Ya-xi,LIU Yan-xin,ZHENG De-xian. Effect of miR-146a on IL-18 expression in mouse macrophage[J]. Chinese journal of cellular and molecular immunology, 2011, 27(5): 477-479 |
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| Authors: | XIANG Yang JIANG Ming-hong WANG Dong-sheng GAO Jing ZHANG Ya-xi LIU Yan-xin ZHENG De-xian |
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| Affiliation: | XIANG Yang,JIANG Ming-hong,WANG Dong-sheng,GAO Jing,ZHANG Ya-xi,LIU Yan-xin,ZHENG De-xian National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005,China |
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| Abstract: | AIM: To investigate the effect of miR-146a on the Th1/Th2 cytokine expression in mouse RAW264.7 cell line and primary peritoneal macrophage.METHODS: miR-146a mimics,mimics negative control(NC mimics),inhibitor miR-146a and inhibitor negative control(NC inhibitor) were transfected into RAW264.7 cells and freshly isolated peritoneal macrophage.IL-18,IL-5 and IL-10 expressions in the cells were measured by real time PCR.RESULTS: MiR-146a mimics suppressed IL-18 expression(P<0.05),and miR-146a specific inhibito... |
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| Keywords: | miR-146a |
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