GM-1治疗脊髓损伤的实验研究

为评价单唾液酸四己糖神经节苷脂对急性脊髓损伤的治疗效果，选取５５只ＳＤ大鼠随机分成对照组，损伤组和治疗和Ａｌｌｅｎ氏法致伤大鼠Ｔ３－Ｌ１脊髓节段。治疗组伤后１５、９０、１８０ｍｉｎ，第２个及第３个２４ｈ分别腹腔内注射ＧＭ－１ １０ｍｇ／ｋｇ，损伤且伤后注射生理盐水。

Experimental Study on Treatment of Acute Spinal Cord Injury with GM-1 in Rats

The therapeutic effects of monosialutetrahexosyl ganglioside (GM-1) in rats with acute spinal cord injury (ASCI) were assessed. Fifty-five rats were randomly divided into control group and injured group A and injured group B. Allen method was used to injury the segments of T3-L1 of the rats. In injured group A, GM-1 (10 mg/kg)was injected intraperitoneally at 15 min. 90 min, 180 min, the second day and third day after trauma. Injured group B and control group were subjected to the injection of normal saline. One rat in each time point of 1 h, 6 h, 24 h, one week and six weeks after trauma in injured groups A and B underwent light and electron microscopic examination of the injured spinal cord tissue. Revlin test was per formed to evaluate motor function of the rats. The results showed that in injured group A the hemorrhage and necrosis of the grey matter were a1leviated, the surrounding white matter survived, capillaries and oligodentrocytes abundantly proliferated, and motor function gradually recovered. It was concluded that GM-1 could improve the spinal cord microcirculation, decrease the secondary patho1ogical damage markedly. and promote the recovery of motor function in the rats with ASCI.