HPLC法测定党参中5-羟甲基糠醛的含量

目的：建立党参中己糖降解产物5-羟甲基糠醛（5-HMF）的含量测定方法，对党参药材中的5-羟甲基糠醛进行含量测定。方法：采用Agilent Extend-C18（250 mm＆#215;4.6 mm，5μm）色谱柱，以乙腈-0.3%醋酸溶液（3∶97）为流动相，检测波长284 nm，流速1.0 mL/min，柱温30℃。结果：5-羟甲基糠醛在浓度为2.538～63.45μg/mL范围内与峰面积具有良好的线性关系（r=0.9998），精密度和重复性的RSD分别为0.8％和2.5%。结论：该方法操作简便，结果准确可靠，可用于党参中5-羟甲基糠醛的含量测定，为进一步评价党参的安全性提供参考。

Determination of the Contents of 5-hydroxymethylfurfural in DangShen by HPLC

Objective：To establish the determination method for 5-hydroxymethylfurfural （5-HMF） in Dang-Shen （Radix Codonopsis Pilosulae） and determine its contents. Methods：Agilent Extend-C18 （250 mm＆#215;4.6 mm, 5μm） chromatographic column was adopted with the mobile phase of acetonitrile-0.3% acetum （3：97）, detection wave-length was 284nm, flow rate was 1.0mL/min, column temperature was 30℃. Results：It showed better linear rela-tionship in the range between 2.538 and 63.45μg/mL and peak area （r=0.999 8）, accuracy and repeatability RSD were 0.8%and 2.5%respectively. Conclusion：The method, simple and reliable, could be used to the determination of 5-HMF contained in DangShen, which could provide reference for further assessment of the safety of DangShen.