睾酮对去势与非去势小鼠前列腺增生影响的实验研究

目的:探讨睾酮(T)对去势和非去势小鼠前列腺增生影响的各自特点及差异。方法:选用48只BALB/c小鼠,随机分为去势对照组(A)、非去势对照组(B)、去势小剂量T组(C)、非去势小剂量T组(D)、去势大剂量T组(E)、非去势大剂量T组(F),每组8只。C、D组和E、F组分别采用12.5mg/(kg.d)和125mg/(kg.d)丙酸睾酮连续腹腔内注射20d,A、B组则注射生理盐水。第21d所有小鼠摘眼法采血后处死,观察前列腺并称重,对比分析其各自病理学特点。结果:A组可见前列腺萎缩及部分脂肪组织替代,B组是正常前列腺。C、D组小鼠前列腺均出现增生,以腹侧叶为主,背侧叶增生不明显,但D组前列腺增生较C组更明显(P<0.05),呈单侧腹侧叶结节状非对称局限性增生。E、F组前列腺增生的程度稍高于C、D组,但并不与T增加的剂量成正比,且伴有腺体局部糜烂坏死和轻度异型。T治疗后小鼠血清T和血管内皮生长因子升高,雌二醇下降,以非去势大剂量T组更明显。结论:去势与非去势小鼠经不同剂量T短期治疗后均可出现前列腺增生,但增生程度和特点存在差别,有助于我们进一步研究睾丸去势、雄激素与前列腺疾病之间的关系。

Testosterone Induces Different-featured Prostate Hyperplasia in Castrated and Uncastrated Mice

Objective： To study the different features of hyperplasia in castrated and uncastrated mice after testosterone （T） treatment. Methods ： Forty-eight BALB/c mice were randomly divided into 6 groups of 8 in each： castrated （ A）, uneastrated （ B）, castrated ＋ low T （ C）, uneastrated ＋ low T （D） , castrated ＋ high T （ E）, uneastrated ＋ high T （F）. Groups C and D were treated with testosterone solution at the dose of 12.5 mg/ （kg·d） and Groups E and F at 125 mg/ （kg·d） for 20 consecutive days, while Groups A and B received saline only. All the mice were sacrificed on the 21st day, their ventral and dorsal prostate glands weighed and their pathological features studied. Results： Atrophic prostates were observed in Group A, but normal in Group B; prostatic hyperplasia was found in both Group C and D, but more obvious in the latter （ P 〈 0.05 ） ; and a slightly higher degree of hyperplasia was noted in Groups E and F than in C and D. There was an increase in serum T and vascular endothelial growth factor （VEGF） concentration and a decrease in serum estrogen （E2） concentration in the testosterone treated groups. Conclusion ： Both castrated and uncastrated mice develop prostate hyperplasia after short-term testosterone treatment, although in different degrees and with different features, which may help further the studies on the assoccation of castration and androgen with prostate diseases.