| 内皮抑素30肽对SGC-7901细胞增殖活性的影响 |
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| 引用本文： | 杨柳,李淑艳. 内皮抑素30肽对SGC-7901细胞增殖活性的影响[J]. 齐齐哈尔医学院学报, 2012, 33(18): 2437-2438 |
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| 作者姓名： | 杨柳 李淑艳 |
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| 作者单位： | 齐齐哈尔医学院医学技术学院;齐齐哈尔医学院生物化学教研室 |
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| 基金项目： | 黑龙江省教育厅基金资助项目12511619 |
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| 摘 要： | 目的研究经RGD修饰的人内皮抑素(endostatin)N-端的30肽对胃癌SGC-7901细胞增殖能力的影响。方法人工合成人内皮抑素1～30位氨基酸(30肽,25～31序列由RGIR GAD改为RGDRGD)所对应的核苷酸序列,连接到质粒pTYB2中,再转化至大肠埃希菌BL21(DE3)中表达,用WST-1试剂检测30肽对SGC-7901细胞增殖活性的影响。结果 30肽能够有效抑制SGC-7901细胞的增殖,并呈时间剂量依赖性。结论 30肽能有效抑制SGC-7901细胞的增殖,其在临床胃癌的治疗上具有潜在的应用前景。
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| 关 键 词： | 内皮抑素 RGD序列 增殖 SGC-7901细胞 |
Experimental study on the role of Peptide 30 Derived from Endostatin in the proliferation process of SGC-7901 cells |
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| Affiliation: | YANG Liu,et al.(Medical Imaging Specialty,Medical Technology School,Qiqihar Medical University,Qiqihar,Heilongjiang 161006,the P.R.China.) |
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| Abstract: | Objective The effect of a 30-amino-acid N-terminal peptide of endostatin(aa 1-30,peptide 30) modified by RGD on proliferation of SGC-7901 cells was studied.Methods The nucleotide sequence encoding amino acids 1-30 of endostatin(peptide 30,with amino acids 25-31 mutated from RGIRGAD to RGDRGD) was artificially synthesized and cloned into the plasmid pTYB2 and expressed in E.coli(DE3).The proliferation of SGC-7901 was observed by WST-1 kit.Results SGC-7901 cell viability was significantly inhibited in a time-and-dose dependent response by peptide 30.Conclusions Peptide 30 effectively suppressed the proliferation of SGC-7901 cells.Peptide 30 can potentially be used to treat gastric carcinoma in clinic. |
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| Keywords: | Endostatin RGD motif Proliferation SGC-7901 cells |
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