T淋巴细胞亚群和铁蛋白联合检测在强直性脊柱炎患者中的初步应用

目的 探讨T淋巴细胞亚群和铁蛋白联合检测在强直性脊柱炎(AS)中的临床价值.方法 用CD4/CD8/CD3三色抗体分别标记AS组、疾病对照组和健康对照组的外周抗凝血,分析CD4+、CD8+的百分率及CD4+/CD8+的比值.再用免疫荧光法分别检测AS组、疾病对照组和健康对照组的外周抗凝血铁蛋白.结果 AS组CD4+百分率和CD4+/CD8+比值均明显低于健康对照组(P<0.05),CD8+百分率明显高于健康对照组(P<0.05);疾病对照组CD4+和CD8+百分率明显低于健康对照组(P<0.05)、CD4+/CD8+比值明显高于健康对照组(P<0.05).AS组的CD8+百分率明显高于疾病对照组(P<0.05),CD4+/CD8+比值明显低于疾病对照组(P<0.05).铁蛋白检测结果AS组明显高于疾病对照组和健康对照组(P<0.05).98例AS患者的铁蛋白与CD8+检测值呈正相关(r=0.846 9,P<0.05),与CD4+及CD4+/CD8+比值呈负相关(r分别为-0.748 7、-0.582 0,P<0.05).结论 通过对T淋巴细胞亚群和铁蛋白联合检测缩短了检测时间,有助于AS的早期诊断、早期治疗.

Application of combined detection of T lymphocyte subsets and ferritin in patients with ankylosing spondylitis

Objective To investigate the clinical value of T-lymphocyte subsets and ferritin detected in patients with ankylosing spondylitis(AS).Methods Anti-coagulated peripheral blood samples of AS group,disease control group and healthy control group were labeled with CD4/CD8/CD3 three-color antibodies.The percentage of CD4+ and CD8+ and CD4+/CD8+ ratio were analyzed.Enzyme-linked fluorescence were performed for the detection of ferritin in all groups.Results Percentage of CD4+ and CD4+/CD8+ ratio in AS group were lower,but percentage of CD8+ was higher than in healthy control group(P<0.05).Percentages of CD4+and CD8+ in disease control group were lower,but CD4+/CD8+ ratio was higher than in healthy control groups(P<0.05).Percentage of CD8+ in AS group was higher,but CD4+/CD8+ ratio was lower than in diseases control group(P<0.05).Ferritin level in AS group was significantly higher than in disease control group and healthy control group(P<0.05).In 98 cases of patients with AS,the ferritin level was positively correlated with CD8+ level(r=0.846 9,P<0.05),and negatively correlated with CD4+ level and CD4+/CD8+ ratio,withrof-0.748 7 and-0.582 0,respectively(P<0.05).Conclusion Combined detection of T lymphocyte subsets and ferritin might be helpful for the early diagnosis and treatment of AS.