氯化三乙基锡对大鼠C6胶质瘤细胞增殖抑制作用

目的：探讨氯化三乙基锡（TETC）对体外培养大鼠C6胶质瘤细胞增殖的抑制作用。方法：采用四甲基偶氮唑盐（MTT）比色法及倒置显微镜观察方法检测0.5、1.0和2.0 μmol·L^-1 TETC对体外培养大鼠C6胶质瘤细胞作用48 h增殖抑制作用，采用电镜方法观察给予0.5、1.0和2.0 μmol·L^-1 TETC 48 h后C6胶质瘤细胞超微结构变化。结果：0.5、1.0和2.0 μmol·L^-1 TETC在体外均可抑制C6胶质瘤细胞增殖，抑制率分别为15.62%、36.16%和41.92%，存在着剂量依赖性上升趋势，抑制率在不同浓度组间以及不同浓度组与对照组间比较，差异均有显著性（P<0.05）。电镜观察0.5、1.0和2.0 μmol·L^-1 TETC作用于C6胶质瘤细胞48 h后其超微结构变化，可见到核内染色质趋边凝聚，排列于核膜内侧，呈早期凋亡改变细胞。结论：TETC可抑制体外培养大鼠C6胶质瘤细胞增殖。

Inhibitory effect of triethyltin chloride on proliferation of rat C6 glioma cells

Objective To study the inhibitory effect of triethyltin chloride(TETC) on the proliferation of cultured rat C6 glioma cells.Methods MTT assay and light microscope were performed to evaluate the inhibitory effect of 0.5,1.0 and 2.0 μmol·L~(-1) TETC on the proliferation of rat C6 glioma cells for 48 h,and electron microscope was performed to observe the ultrastructure changes of C6 glioma cells treated by 0.5,1.0 and 2.0 μmol·L~(-1)TETC for 48 h.Results 0.5,1.0 and 2.0 μmol·L~(-1) TETC could inhibit the proliferation of cultured C6 glioma cells in vitro,and the inhibitory rate were 15.62%,36.16% and 41.92%,respectively.The inhibitory rate of TETC on the proliferation of C6 glioma cells evaluated by MTT assay increased in a dose-dependent manner,and there was significant difference compared with control and compared with each other among every two adjacent-dose groups(P<0.05).The nuclear chromatic condensation and margination were found in C6 glioma cells treated by 0.5,1.0 and 2.0 μmol·L~(-1) TETC for 48 h by electron microscope,which showed the early ultrastructure changes of apoptosis.Conclusion TETC can inhibit the proliferation of cultured rat C6 glioma cells in viro.